NCI-H1975 and HCC827 cells were seeded at 500 cells per well of 6-well plates in a medium containing Penicillin-Streptomycin-Gentamicin Solution (Solarbio, P1410). After 24 h of incubation, the cells were treated with different concentrations of CNC every 5 days until colonies formed in 10 days. The remaining colonies were stained with crystal violet.
Penicillin streptomycin gentamicin solution
Manufactured by Solarbio
Sourced in China
Penicillin-Streptomycin-Gentamicin Solution is a sterile, ready-to-use liquid medium containing a combination of antibiotics commonly used in cell culture applications to prevent bacterial contamination.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Procell (2 mentions)
Lipofectamine 2000 transfection kit, by Thermo Fisher Scientific (1 mentions)
Dmem f12, by Wuhan Servicebio Technology (1 mentions)
Sirna, by GenePharma (1 mentions)
Sirna1, by GenePharma (1 mentions)
Nc sirna, by GenePharma (1 mentions)
Paraformaldehyde (pfa), by Beyotime (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin gentamicin solution, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Dmem high glucose medium, by Procell (1 mentions)
Huvecs, by Procell (1 mentions)
Huvecs, by iCell Bioscience (1 mentions)
Penicillin streptomycin solution, by Procell (1 mentions)
High glucose dmem, by Procell (1 mentions)
Hibepic, by ScienCell (1 mentions)
Phorbol 12 myristate 13 acetate, by Merck Group (1 mentions)
Ifn γ, by R&D Systems (1 mentions)
Lipopolysaccharides (lps), by Solarbio (1 mentions)
7 protocols using penicillin streptomycin gentamicin solution
1
CNC Inhibits Cancer Cell Colony Formation
2
Silencing ADM and POLR1D in OSCC Cells
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The OSCC cell lines CAL-27 (Servicebio, Wuhan, China) and SAS (Cellcook, Guangzhou, China) were cultivated in Dulbecco Modified Eagle Medium F12 (DMEM/F12) (Servicebio, Wuhan, China) supplemented with 10% fetal bovine serum (FBS) (ABW, Shanghai, China) and 100 U/mL penicillin-streptomycin-gentamicin solution (Solarbio, Wuhan, China). Culturing was performed in a CO2-controlled incubator at 37 °C. A density of 1 × 106 cells was seeded in T-25 cell culture flasks. For transfection, siRNAs targeting ADM (siRNA1-ADM, siRNA2-ADM, siRNA3-ADM), siRNAs targeting POLR1D (siRNA1-POLR1D, siRNA2-POLR1D, siRNA3-POLR1D), and a negative control (siRNA-NC) (GenePharma, Shanghai, China) were transfected into cells using the Lipofectamine™ 2000 transfection kit (Life Technologies, Carlsbad, CA, USA). After 24 h, the expression levels of ADM and POLR1D were assessed via RT-qPCR.
3
EHEC Infection in Caco-2 Cells
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Caco-2 cells were seeded into 12-well plates at approximately 5 × 104 cells/well 1 day prior to infection. The next day, cells were infected with EHEC, ΔespF, and ΔespF/pespF for 3 and 6 h. The non-adhered bacteria were aspirated, and the medium was replaced with a growth medium supplemented with Penicillin-Streptomycin-Gentamicin Solution (Solarbio). Cultures were continued for 96 h and media were replenished as necessary. At each time point, cell numbers were estimated based on the optical density at 595 nm (OD595) of solubilized crystal violet from 4% paraformaldehyde (Beyotime)-fixed cells.
4
Culturing IBRS-2 Cells for Foot-and-Mouth Disease
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IBRS-2 cells (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Grand Island, NY, United States) supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin–streptomycin–gentamicin solution (Solarbio, Beijing, China) at 37°C with 5% CO2 in 75 cm2 cell culture flasks. SVA strain A/ZJ/2015 was isolated and preserved at the OIE/National Foot-and-Mouth Disease Reference Laboratory (Lanzhou, Gansu, China).
5
Porcine Macrophage and Cell Culture Methods
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PAMs were prepared by bronchoalveolar lavage, and porcine bone marrow-derived macrophages (BMDMs) were prepared from bone marrow according to previously published methods (52 (link), 53 (link)) and grown in Roswell Park Memorial Institute 1640 (Solarbio Life Science, China) medium containing 1% penicillin-streptomycin-gentamicin solution (Solarbio Life Science, China) and 10% porcine serum. HEK293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen, Waltham, MA, USA) supplemented with 1% penicillin-streptomycin-gentamicin solution and 10% fetal bovine serum. All cells were cultured at 37°C under 5% CO2 atmosphere saturated with water vapor. ASFV-WT was propagated on PAMs as previously described (54 (link)) and stored at −80°C.
6
Culturing Human Endothelial Cells
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Human umbilical vein endothelial cells (HUVECs) and HemECs were both obtained from iCell Bioscience Inc (Shanghai, China). HUVECs and HemECs were cultured in high-glucose DMEM medium (Procell, Wuhan, China) supplemented with 10% fetal bovine serum (Procell, Wuhan, China) and 1% penicillin–streptomycin-gentamicin solution (Solarbio, Beijing, China). All cells were cultivated in a humid environment with 5% CO2 at a temperature of 37 °C.
7
Cholangiocarcinoma Cell Lines and Macrophage Polarization
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Two human cholangiocarcinoma cell lines (HUCCT1 and QBC939) were purchased from the Cell Bank of the Shanghai Institute for Biological Sciences (Chinese Academy of Sciences, Shanghai, China). Human intrahepatic biliary epithelial cells (HIBEpiCs) were purchased from ScienCell (ScienCell, USA). The cell lines were cultured in high glucose-DMEM (Procell, China) supplemented with 10 % fetal bovine serum (FBS; Biological Industries, Israel) and 1 % Penicillin-Streptomycin-Gentamicin Solution (Solarbio, China). Human monocytic THP-1 (Procell) cells were cultured in Roswell Park Memorial Institute medium (Procell) culture medium with 10 % of heat inactivated FBS (Procell) and supplemented with 0.05 mM β-mercaptoethanol and 1 % Penicillin-Streptomycin Solution (Procell). THP-1 monocytes were differentiated into macrophages by incubation with 150 nM phorbol 12-myristate 13-acetate (Sigma, Germany) for 24 h, followed by incubation in the original medium for 24 h. Then it was incubated with 20 ng/mL IFN-γ (R&D Systems, USA) and 10 pg/mL LPS (Solarbio) to polarize it from M0–M1. They were cultured together in a humidified incubator at 37 °C with 5 % CO2. Cells were treated with filtered bile containing varying concentrations of amylase or bile containing varying concentrations of IL-8.
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