Paraffin-embedded and formalin-fixed HCC samples were cut into 5-μm sections, which were then processed for immunohistochemistry as previously described. Briefly, the sections were incubated with Abs against the following human antigens: CD8 (rabbit monoclonal, clone EP334, ZSBio, China), CD11b (rabbit monoclonal, clone EPR1344, Abcam, UK), CD68 (mouse monoclonal; clone PG-M1; Dako Cytomation, USA), CD15 (mouse monoclonal; clone LeuM1; ZSBio), CD206 (mouse monoclonal, clone 685645, R&D Systems, USA), CD204 (mouse monoclonal, clone SRA-C6, Transgenic, Japan), CD163 (mouse monoclonal, clone 10D6, ZSBio) and CD169 (sheep polyclonal, clone NS0, R&D Systems). Then, the sections were stained in an EnVision System (Dako Cytomation, USA). Positive cells were detected by microscopy and quantified using the Vectra-Inform image analysis system (Perkin-Elmer Applied Biosystems, Foster City, CA, USA).
Leum1
Manufactured by ZSGB-BIO
Sourced in United States
The LeuM1 is a laboratory equipment designed for the detection and analysis of leukocytes (white blood cells). It utilizes advanced optical and electronic technologies to provide accurate and reliable measurements of various leukocyte parameters.
Automatically generated - may contain errors
Lab products found in correlation
Envision system, by Agilent Technologies (2 mentions)
Epr1344, by Abcam (2 mentions)
Pg m1, by Agilent Technologies (2 mentions)
Cd206, by R&D Systems (2 mentions)
Ep334, by ZSGB-BIO (2 mentions)
Cd163, by ZSGB-BIO (2 mentions)
Cd169, by R&D Systems (2 mentions)
Vectra inform image analysis system, by Thermo Fisher Scientific (2 mentions)
Cd11b, by Abcam (2 mentions)
2 protocols using leum1
1
Immunohistochemical Analysis of Immune Cells in HCC
2
Immunohistochemical Analysis of Immune Cells in HCC
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Paraffin-embedded and formalin-fixed HCC samples were cut into 5-μm sections, which were then processed for immunohistochemistry as previously described. Briefly, the sections were incubated with Abs against the following human antigens: CD8 (rabbit monoclonal, clone EP334, ZSBio, China), CD11b (rabbit monoclonal, clone EPR1344, Abcam, UK), CD68 (mouse monoclonal; clone PG-M1; Dako Cytomation, USA), CD15 (mouse monoclonal; clone LeuM1; ZSBio), CD206 (mouse monoclonal, clone 685645, R&D Systems, USA), CD204 (mouse monoclonal, clone SRA-C6, Transgenic, Japan), CD163 (mouse monoclonal, clone 10D6, ZSBio) and CD169 (sheep polyclonal, clone NS0, R&D Systems). Then, the sections were stained in an EnVision System (Dako Cytomation, USA). Positive cells were detected by microscopy and quantified using the Vectra-Inform image analysis system (Perkin-Elmer Applied Biosystems, Foster City, CA, USA).
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