A high-performance size-exclusion chromatography (HPSEC) system (Model 1525 binary HPLC pump, equipped with a Model 2414 refractive index detector, Waters Co., Milford, MA, USA) was used to estimate the molecular weight distribution in the extracts and their fractions and quantify the yield of polysaccharide extraction. Columns TSK G5000 PWXL and TSK G3000 PWXL (Tosoh Co., Yamaguchi, Japan) were used in series with isocratic flow rate of 0.4 mL/min of 0.1 M sodium chloride. Detector was operated at 30 °C. Dextrans (50–670 kDa) and soybean rhamnogalacturonan (0.3–5 mg/mL) were used as standards for calibration.
Model 1525 binary hplc pump
Manufactured by Waters Corporation
Sourced in United States
The Waters Model 1525 binary HPLC pump is a high-performance liquid chromatography pump designed to deliver precise and accurate flow rates for a variety of analytical applications. It features two independent solvent delivery systems, allowing for the mixing of up to two solvents. The pump is capable of generating flow rates up to 10 mL/min with a maximum pressure of 6000 psi.
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2 protocols using model 1525 binary hplc pump
1
Polysaccharide Molecular Weight Analysis
2
Molecular Weight Analysis of Carbohydrates
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A high-performance size-exclusion chromatography (HPSEC) system (model 1525 binary HPLC pump, equipped with a model 2414 refractive index detector, Waters Co., Milford, MA, USA) was used to estimate the molecular weight distribution of the carbohydrates. Analysis was determined using a TSK G5000 PWXL (Tosoh Co., Yamaguchi, Japan) column, with dextrans (50–670 kDa), soybean rhamnogalacturonan (0.125–1 g/L), and galactoglucomannan (0.125–1 g/L) standards for molecular weight and concentration calibration. The temperature of the system was 30 °C, the eluent was 0.1 M NaCl, and the flow rate was set at 0.4 mL/min.
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