Acetylated histone h3

Western blotting was performed as described previously 17 (link). The primary antibodies against acetylated histone H3, acetylated histone H4, and β-actin were purchased from Santa Cruz Biotechnology (CA, USA). The primary antibodies against p21, p27, cdc25C, cyclin B1, Bim, Bax, Bcl-2, Bcl-xL, RAGE, and LC3 were purchased from Epitomics (CA, USA). The primary antibodies against p-cdc25C, cdc2, p-cdc2, Cyto-C, cleaved caspase-9, cleaved caspase-3, cleaved caspase-7, cleaved-PARP, PARP, PI3KC3, Beclin 1, and p62 were purchased from Cell Signaling Technology (MA, USA). All secondary antibodies were purchased from Jackson Immuno Research Laboratories (PA, USA).

5-FU, oxaliplatin (L-OHP), and propidium iodine (PI) were purchased from Sigma-Aldrich, Munich, Germany; MS-275 and MERCK60 were from Selleck Chemicals, Munich, Germany; and TO-PRO-3 was from Life Technologies, Ober-Olm, Germany. Antibodies for immunoblot were from: Millipore, Darmstadt, Germany (HDAC1 (05–100); acetylated histone H3 (06–599)), Santa Cruz Biotechnology, Heidelberg, Germany (HDAC2 (sc-7899); ɣH2AX (sc-101696)), Enzo Life Sciences, Lörrach, Germany (HSP90 (ADI-SPA-830)), Novus Biologicals, Heidelberg, Germany (p-KAP1 (Ser824), NB100-2350), Abcam, Cambridge, UK (p-ATM (Ser1981), ab81292; HDAC3, ab16047), Cell Signaling Technology, Frankfurt/Main, Germany (ATM (cs-2873); p-AKT1/p-AKT2/p-AKT3 (cs-9271; Ser473); CHK1 (cs-2360); HDAC6 (cs-7558); anti-p-Ser15-p53 (cs-9284); TS (cs-9045)), Sigma-Aldrich, Munich, Germany (acetylated tubulin (6-11B-1), T7451), BD Biosciences, Heidelberg, Germany (β-catenin (BD610154)), Thermo Fisher Scientific, Frankfurt/Main, Germany (RRM2 (PA5-13,570); vimentin (V9), MS-129-P). Antibodies against HDAC2 (#5113) from Cell Signaling Technology, Frankfurt/Main, Germany, and HDAC1 (05–100) from Millipore, Darmstadt, Germany, were used for immunofluorescence analysis.