Transwell cell culture chamber filters 8 μm pore

Cell migration was examined in Transwell cell culture chamber filters (8 μm pore) (Corning, New York, NY, USA). Green tracker fluorescent-labeled cells were seeded at 5  ×  10⁴ cells in RPMI-0.2% FBS. Following 16 h incubation, the invading cells were fixed and analyzed by fluorescence microscopy and counted. Images were processed and analyzed using FIJI Image.

Proliferation assays were performed using AlamarBlue Cell Viability Reagent (Thermo Fisher, Waltham, MA, USA). Around 6000 cells/well were plated in a 96-well plate in 100 μL medium and starved for 12 h. After that time, 10 μL of AlamarBlue Reagent was added and incubated in the dark at 37 ℃ for 12 h. Absorbance was read at 540 and 620 nm. Cell migration was examined in Transwell cell culture chamber filters (8 μm pore) (Corning). Cells were seeded at 5 × 10⁴ cells in DMEM with 0.2% FBS. Following 48 h incubation, the invading cells were fixed and analyzed by fluorescence microscopy and counted. Images were processed and analyzed using Fiji Image.