Rabbit anti reck

Manufactured by Cell Signaling Technology

Rabbit anti-Reck is a primary antibody product that targets the Reck (reversion-inducing cysteine-rich protein with Kazal motifs) protein. Reck is a membrane-anchored glycoprotein that acts as a negative regulator of matrix metalloproteinases (MMPs), which are enzymes involved in the breakdown of the extracellular matrix. The Rabbit anti-Reck antibody can be used to detect and study the Reck protein in various experimental applications.

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Lab products found in correlation

Isolectin gs ib4, by Thermo Fisher Scientific (2 mentions) Rat anti mouse pecam cd31, by BD (2 mentions) Rabbit anti glut1, by Thermo Fisher Scientific (2 mentions) Rat anti alpha tubulin, by Thermo Fisher Scientific (2 mentions) Rabbit anti 6xmyc, by Cell Signaling Technology (1 mentions) Texas red streptavidin, by Vector Laboratories (1 mentions) Rabbit anti ha, by Cell Signaling Technology (1 mentions) Mouse anti actin, by Merck Group (1 mentions) Rat anti mouse plvap meca 32, by BD (1 mentions)

2 protocols using rabbit anti reck

Antibody-based Tissue and Protein Analysis

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The following antibodies were used for tissue immunohistochemistry: rat anti-mouse PECAM/CD31 (BD Biosciences 553370); rabbit anti-GLUT1 (Thermo Fisher Scientific RB-9052-P1). Alexa Fluor-labeled secondary antibodies and GS Lectin (Isolectin GS-IB4) were from Thermo Fisher Scientific.
The following antibodies were used for immunoblot and immuno-staining analysis: mouse anti-1D4 (MacKenzie et al., 1984 (link)); rabbit anti-6xMyc (JH6204); rabbit anti-Reck (Cell Signaling 3433); rat anti-alpha tubulin (Invitrogen MA1-80017); and AP-conjugated horse anti-mouse IgG antibody (Vector Laboratories AP-2000). Fluorescent secondary antibodies for immunoblotting were from Li-Cor.

Cho C., Wang Y., Smallwood P.M., Williams J, & Nathans J. (2019). Molecular determinants in Frizzled, Reck, and Wnt7a for ligand-specific signaling in neurovascular development. eLife, 8, e47300.

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Comprehensive Immunohistochemistry and Western Blot Techniques

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The following antibodies were used for tissue immunohistochemistry: rat anti-mouse PECAM/CD31 (BD Biosciences 553370); rabbit anti-Glut1 (Thermo Fisher Scientific RB-9052-P1); rat anti-mouse PLVAP/MECA-32 (BD Biosciences 553849); mouse anti-Claudin5, Alexa Fluor 488 conjugate (Thermo Fisher Scientific 352588); Texas Red streptavidin (Vector Laboratories SA-5006); rabbit anti-GFP, Alexa Fluor 488 conjugate (Thermo Fisher Scientific A21311); rabbit anti-6×Myc (JH6204); Brilliant Violet 421 rat anti-mouse/human Cd11b (BioLegend 101235); and rat anti-F4/80, Alexa Fluor 488 conjugate (Bio-Rad MCA497A488T). Alexa Fluor-labeled secondary antibodies and GS Lectin (Isolectin GS-IB4) were from Thermo Fisher Scientific.
The following antibodies were used for immunoblot analysis: rabbit anti-6×Myc (JH6204); rabbit anti-HA (Cell Signaling 3724); mouse anti-actin (EMD Millipore MAB1501); rabbit anti-Reck (Cell Signaling 3433); mouse anti-1D4 (MacKenzie et al., 1984 (link)); and rat anti-alpha tubulin (Invitrogen MA1-80017). Fluorescent secondary antibodies for immunoblotting were from Li-Cor.

Cho C., Smallwood P.M, & Nathans J. (2017). Reck and Gpr124 are Essential Receptor Cofactors for Wnt7a/Wnt7b-Specific Signaling in Mammalian CNS Angiogenesis and Blood-Brain Barrier Regulation. Neuron, 95(5), 1056-1073.e5.

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