Thapsigargin bml pe180

5-FU (F6627, Sigma), Azacytidine, (A2385, Sigma), Etoposide (E1383, Sigma), Fluoforte (ENZ-2204, ENZO), Gemcitabine (G6423, Sigma), Hoechst 33342 #4082, Cell Signaling), Mitotracker (M22426, Invitrogen), Thapsigargin (BML-PE180, Enzo), Tunicamycin (T7765, Sigma), A23817 (C7522, Sigma), Staurosporine (S4400, Sigma), Histamine (H7125, Sigma).

All chemicals were reagent grade and were purchased from Sigma/Aldrich (St. Louis, MO) unless otherwise indicated. Fetal bovine lipoprotein-deficient serum (LPDS) was obtained from Intracel (Frederick, MD, United States) or Sigma (S5394). U18666A (3-β-[2-(diethylamino)ethoxy]androst-5-en-17-one) (BML-S200; Enzo Life Sciences, Switzerland) was dissolved in ethanol and stored as a 10 mM stock solution at −20°C. 25-hydroxycholesterol (25-HC) (H1015; Sigma/Aldrich) was dissolved in ethanol as a 10 mg/ml stock solution and stored at −20°C. Tunicamycin (T7765; Sigma/Aldrich) was dissolved in DMSO as a 4 mg/ml stock solution and stored at −20°C. Thapsigargin (BML-PE180; Enzo Life Sciences) was dissolved in DMSO as a 1 mM stock solution and stored at −20°C. Brefeldin A (B6542; Sigma/Aldrich) was dissolved in DMSO as 10 mg/ml stock solution and stored at −20°C.