To specifically analyze the expression of the C2orf40, IHC was performed on the paraffin-embedded tumor tissue sections. Briefly, after deparaffinization and rehydration, antigen retrieval was undertaken using citrate solution. The primary antibody (dilution, 1:400; Abcam) was added and incubated overnight at 4 ℃, and a secondary antibody was added and incubated for 30 min in a humid chamber, followed by washing with Tris-buffered saline (TBS) buffer. Slides were then incubated for 30 min with EnVision peroxidase reagent (DAKO, Carpentaria, CA, USA). Finally, the slides were stained with 3, 3-diaminobenzidine (DAB) for 5 min, and Mayer’s hematoxylin solution was used for counterstain. The percentage of positively stained NPC cells in three images was assessed using the ImageJ software as previously described [20 (link)].
Envision peroxidase reagent
Manufactured by Agilent Technologies
Sourced in United States
The EnVision peroxidase reagent is a laboratory product designed for use in various analytical techniques. It serves as a detection reagent that utilizes peroxidase enzyme activity to facilitate the visualization of target analytes. The core function of this reagent is to provide a reliable and sensitive means of signal amplification during immunoassay and other similar applications.
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Lab products found in correlation
2 protocols using envision peroxidase reagent
1
Immunohistochemical Analysis of C2orf40 in NPC
2
Immunohistochemical Analysis of C2orf40 Expression
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To speci cally analyze the expression of the C2orf40, IHC was performed on the para n-embedded tumor tissue sections. Brie y, after depara nization and rehydration, antigen retrieval was undertaken using citrate solution. The primary antibody (dilution, 1:400; Abcam) was added and incubated overnight at 4 ℃, and a secondary antibody was added and incubated for 30 min in a humid chamber, followed by washing with Tris-buffered saline (TBS) buffer. Slides were then incubated for 30 min with EnVision peroxidase reagent (DAKO, Carpentaria, CA, USA). Finally, the slides were stained with 3, 3diaminobenzidine (DAB) for 5 min, and Mayer's hematoxylin solution was used for counterstain. The percentage of positively stained NPC cells in three images was assessed using the ImageJ software as previously described [20] .
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