Donkey anti sheep igg hrp

Manufactured by Santa Cruz Biotechnology

Donkey anti-sheep IgG HRP is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed to detect and bind to sheep immunoglobulin G (IgG) in various immunoassay and immunohistochemistry applications.

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Lab products found in correlation

Mouse anti glun1, by Merck Group (1 mentions) Rabbit anti glun2a, by Merck Group (1 mentions) Ecltm, by GE Healthcare (1 mentions) Mouse anti actin, by Merck Group (1 mentions) Mouse anti psd95, by Thermo Fisher Scientific (1 mentions) Mouse anti ha, by Santa Cruz Biotechnology (1 mentions) Complete protease inhibitor, by Merck Group (1 mentions) Western blotting luminol reagent, by Santa Cruz Biotechnology (1 mentions) α tubulin, by Santa Cruz Biotechnology (1 mentions) Laemmli sample buffer, by Bio-Rad (1 mentions) Ripa buffer, by Merck Group (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Goat anti mouse igg h l hrp conjugate, by Bio-Rad (1 mentions) Goat anti rabbit igg h l hrp conjugate, by Bio-Rad (1 mentions)

2 protocols using donkey anti sheep igg hrp

Immunoblot Antibody Characterization

Cited 2 times

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Rabbit anti-NPTX1, rabbit anti-NPTX2, mouse anti-NPTX2, and mouse anti-Arc were described previously (29 (link), 53 (link)). All other antibodies are from commercial companies. Sheep anti-NPTXR is from R&D Systems [catalog number: AF4414; research resource identifier (RRID): AB_2153869); mouse anti-PSD95 is from Thermo Fisher Scientific (catalog number: MA1-046; RRID: AB_2092361); mouse anti-GluN1 is from Millipore (catalog number: 05-432; RRID: AB_390129); rabbit anti-GluN2A is from Sigma-Aldrich (catalog number: G9038; RRID: AB_259980); rabbit anti-GluA4 is from Millipore (catalog number: AB1508; RRID: AB_90711); rabbit anti-HA for Western blot is from eBioscience (catalog number: 14-6756-81; RRID: AB_468301); mouse anti-HA for RiboTag pulldown is from Sigma-Aldrich (catalog number: H3663; RRID: AB_262051); mouse anti-actin is from Sigma-Aldrich (catalog number: A2228; RRID: AB_476697); ECL^TM (enhanced chemiluminescence) anti-mouse immunoglobulin G (IgG) horseradish peroxidase (HRP) is from GE Healthcare (catalog number: NA931V); ECLTM anti-rabbit IgG HRP is from GE Healthcare (catalog number: NA934V); donkey anti-sheep IgG HRP is from Santa Cruz Biotechnology (catalog number: sc-2473; RRID: AB_641190); and mouse anti-HA is from Santa Cruz Biotechnology (catalog number: sc7392; RRID: AB_627809).

Xiao M.F., Roh S.E., Zhou J., Chien C.C., Lucey B.P., Craig M.T., Hayes L.N., Coughlin J.M., Leweke F.M., Jia M., Xu D., Zhou W., Conover Talbot C J.r., Arnold D.B., Staley M., Jiang C., Reti I.M., Sawa A., Pelkey K.A., McBain C.J., Savonenko A, & Worley P.F. (2021). A biomarker-authenticated model of schizophrenia implicating NPTX2 loss of function. Science Advances, 7(48), eabf6935.

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Protein Expression Analysis by Western Blot

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Protein lysate was prepared by using RIPA buffer (Sigma, R0278) supplemented with complete protease inhibitor (Sigma). Lysates were centrifuged at 16,000 x g for 15 minutes. The resultant supernatant was mixed with 4x Laemmli sample buffer (Bio-Rad, #161–0747). Total 20 μg protein lysates were separated by SDS-PAGE gel electrophoresis. The proteins were transferred to a polyvinylidene fluoride membrane and blocked in 5% milk at room temperature, and incubated with following primary antibodies diluted in 5% milk overnight at 4°C. Gapdh (Santa Cruz Biotechnology, sc-32233), α-Tubulin (Santa Cruz Biotechnology, sc-8035), myosin (DSHB, MF20), MyoD (Santa Cruz Biotechnology, SC-304), MyoG (DSHB, F5D). The HRP-conjugated secondary antibodies: Donkey anti-sheep IgG-HRP (Santa Cruz Biotechnology, sc-2473), Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, 170–6516) and Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (Bio-Rad, 170–6515) were diluted at 1:5,000. Signal detection was performed using Western Blotting Luminol Reagent (Santa Cruz Biotechnology, sc2048).

Zhang H., Shang R, & Bi P. (2021). Feedback regulation of Notch signaling and myogenesis connected by MyoD–Dll1 axis. PLoS Genetics, 17(8), e1009729.

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