Lncap cell line

Human prostate cancer LNCaP cell line was purchased from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen) GmbH-German Collection of Microorganism and Cell Cultures (Braunschweig, Germany). The LNCaP cells were maintained in monolayer cultures in RPMI 1640 containing 10% fetal bovine serum (FBS), 100 IU/mL penicillin, and 100 µg/mL streptomycin. The cancer cells were grown at 37 °C in an atmosphere with 5% CO₂ and 100% humidity. All reagents for cell culture were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

The research was conducted on three stable cell models of prostate cancer: LNCaP cell line from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH), DU-145 cell line from ATCC (American Type Culture Collection) and PC3 cell line from ECACC (90112714; European Collection of Authenticated Cell Cultures). Cell lines authenticity were confirmed by short-tandem repeat (STR) DNA profiling (LGC Standards Cell Line Authentication Service, Germany; 2014). The androgen-sensitive LNCaP cell line is a model of early, low invasiveness prostate cancer. The androgen-irresponsive DU-145 and PC3 cell lines were models of subsequent malignant stages of prostate cancer. The cells were passaged at least twice after thawing from liquid nitrogen and cultured in a humidified atmosphere at 37 °C with 5% CO₂ in RPMI 1640 (LNCaP, PC3) or DMEM medium (PC3). In addition, standard supplements were used: 10% heat-inactivated Fetal Bovine Serum (FBS), 1 mM Sodium Pyruvate, 10 mM HEPES buffer and antibiotics (penicillin 50 U/ml; streptomycin 50 mg/ml; neomycin 100 mg/ml).