Blood was collected from a Hickman central venous access catheter and placed in a citrated tube between the hours of 8-10 a.m. at baseline (prior to the conditioning regimen), and then weekly through day 100 post-HCT. Blood was centrifuged at 2500 rotations per minute at 4 degrees Celsius for 15 minutes and pl asma was aspirated and frozen (-70°C) in 2 mL aliquots until analysis. At the time of analysis, plasma was rapidly thawed and the concentrations of PAI-1 activity (Chromolize, Biopool, Ventura, CA), t-PA antigen (Asserachrom, Diagnostica Stago, Parsippany, NJ , and D-dimer (Asserachrom, Diagnositca Stago, Parsippany, NJ) were determined by immunoassay. The intra-assay and inter-assay coefficient of variation is 6-8% for these analytes. Normal values were PAI-1 <20.4 IU/mL; tPA 1.8-12.5 ng/mL. and D-dimer <590 μg/mL16 (link)Urine was also collected between the hours of 8-10 a.m., immediately placed on ice, separated into 2 mL aliquots and frozen at -80 degrees until time of analysis.
Urinary albumin was determined using an immunoturbidimetric assay using a Cobas c 11 analyzer in aliquots of untreated urine samples. The inter-assay coefficient of variation (CV) of the assay is 0.7-2.2% and intra-assay CV is 1.0-1.6%. A quantitative determination of urine creatinine was measured on Roche/Hitachi modular automated clinical chemistry analyzers.
Urinary albumin was determined using an immunoturbidimetric assay using a Cobas c 11 analyzer in aliquots of untreated urine samples. The inter-assay coefficient of variation (CV) of the assay is 0.7-2.2% and intra-assay CV is 1.0-1.6%. A quantitative determination of urine creatinine was measured on Roche/Hitachi modular automated clinical chemistry analyzers.