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Sc 376182

Manufactured by Santa Cruz Biotechnology
Sourced in United States

Sc-376182 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is designed for use in scientific research and analysis. The core function of this product is to facilitate specific laboratory procedures, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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2 protocols using sc 376182

1

Western Blot Analysis of Cellular Proteins

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Total protein was resolved by SDS-PAGE (10 to 15% Tris-glycine), transferred onto Hybond nitrocellulose membrane (Amersham Biosciences), and probed with antibodies specific for WRN (D-6; sc-376182, Santa Cruz Biotechnology [SCBT]), SIRT1 (B-7; sc-74465; SCBT), and GAPDH (G-9; sc-365062; SCBT). Western blots were visualized by using species-specific horseradish peroxidase-conjugated secondary antibodies (Sigma) and enhanced chemiluminescence (Thermo/Pierce). Densitometry analysis was performed using ImageJ analysis software (NIH).
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2

Immunoblotting Antibody Panel for Cellular Analyses

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The antibodies used for experiments included antibodies against GFP (1:1000; sc-9996; Santa Cruz Biotechnology, Dallas, TX, USA); GST (1:5000; sc-138; Santa Cruz Biotechnology), actin (1:10,000; sc-47778; Santa Cruz Biotechnology), WRN (1:300; GTX101081; GeneTex and 1:300; sc-376182; Santa Cruz Biotechnology), lamin A/C (1:10,000; sc-376248; Santa Cruz Biotechnology), progerin (1:300; sc-81611; Santa Cruz Biotechnology and ab66587; Abcam, Cambridge, UK), Ki67 (1:400; ab15580; Abcam), H3K9me3 (1:400 and 1:2000; ab8898; Abcam), p16-INK4A (1:300; 10,883–1-AP; Proteintech, Rosemont, IL, USA), cyclin B1 (1:200; sc-594; Santa Cruz), cdc25C (1:500; #4688; Cell Signaling technology) and Rad51 (1:200; #05-530; Millipore).
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