Em cpd300 scanning electron microscope

A total of 40 mg of DOX was dissolved in 10 mL of methyl alcohol, and then the mixed solution was transferred to the SC-CO₂ reactor (10 MPa, 45℃) at a speed of 1 mL/min. Fresh CO₂ was pumped into the high pressure vessel at a constant flow rate of 35 g/min to remove the solvent, resulting in the precipitation of nanoDOX. After the solution was completely injected into the vessel, fresh CO₂ was continued for additional 30 min to completely remove the residual solvent. Then, the collected nanoDOX was characterized by transmission electron microscopy (TEM) on a TecnaiG2 Spirit Electron Microscope (FEI Co., Hillsboro, OR, USA), and dynamic light scattering (DLS) using a Malvern Zetasizer (Malvern Instruments Ltd., Malvern, UK). The analysis of the drug structure was performed by liquid chromatography-mass spectrometry (LC-MS) using a shimadzu high-pressure liquid chromatography (HPLC) system equipped with an SPD-20A ultraviolet-visible (UV-VIS) light detector (Shimadzu., Kyoto, Japan) and scanning electron microscopy (SEM) on a Leica EM CPD300 Scanning Electron Microscope (Leica Microsystems GmbH, Wetzlar, German). The fluorescence imaging of the reporter and fluorescent signal intensity measurements were performed using an IVIS Lumina III Fluorescence Imaging system (Hopkinton, USA). The contact angle detection was performed using a DSA-100 system (Hamburg, German).

A total of 50 mg of ICG was dissolved in 10 mL of absolute ethyl alcohol, and the solution was transferred to the supercritical carbon dioxide (SC-CO₂) reactor. Carbon dioxide (CO₂) was pumped into the high-pressure vessel at a constant flow rate of 35 g/min to remove the ethyl alcohol, resulting in the precipitation of nanoICG. After the solution was injected into the vessel, CO₂ was pumped for an additional 20 min to completely remove the ethyl alcohol (Fig. 1A). The nanoICG was characterized by scanning electron microscopy (SEM) using a Leica EM CPD300 scanning electron microscope (Leica Microsystems GmbH, Wetzlar, German) and dynamic light scattering (DLS) using a Malvern zetasizer (Malvern Instruments Ltd., Malvern, UK). The structure of the drug was carried out by liquid chromatography-mass spectrometry (LC-MS) using a Shimadzu high-pressure liquid chromatography (HPLC) system equipped with an SPD-20 A ultraviolet-visible (UV-VIS) light detector (Shimadzu Corp., Kyoto, Japan) Fluorescent reporter detection and fluorescent signal intensity measurements were performed using an IVIS Lumina II fluorescence imaging system (PerkinElmer Inc., Hopkinton, MA, USA).