[3H]-labeled estrone sulfate (ES) was purchased from PerkinElmer (Waltham, MA, USA). Membrane-impermeable biotinylation reagent Sulfo-NHS-SS-biotin, streptavidin agarose resin, protein G agarose resin, an anti-mouse HRP-linked antibody, and an anti-rabbit HRP-linked antibody were purchased from Thermofisher Scientific (Waltham, MA, USA). A mouse anti-myc antibody (clone 9E10) was bought from Roche (Indianapolis, IN, USA). Rabbit anti-SUMO2/3, anti-Ubc9, and anti-SENP2 antibodies were purchased from Abcam (Cambridge, MA, USA). Topotecan HCL, ginkgolic acid (GA), and 2-D08 were obtained from Selleck Chemicals (Houston, TX, USA). All other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Protein g agarose resin
Manufactured by Thermo Fisher Scientific
Sourced in United States
Protein G agarose resin is a solid-phase affinity chromatography medium used for the purification of antibodies and other proteins. It consists of recombinant Protein G covalently coupled to agarose beads. Protein G has a high affinity for the Fc region of immunoglobulins, allowing for the selective capture and isolation of antibodies from complex sample matrices.
Automatically generated - may contain errors
Lab products found in correlation
Anti ubc9, by Abcam (1 mentions)
Streptavidin agarose resin, by Thermo Fisher Scientific (1 mentions)
Rabbit anti sumo2 3, by Abcam (1 mentions)
Topotecan hcl, by Selleck Chemicals (1 mentions)
3h labeled estrone sulfate, by PerkinElmer (1 mentions)
Anti senp2, by Abcam (1 mentions)
Sulfo nhs ss biotin, by Thermo Fisher Scientific (1 mentions)
Akta pure protein purification system, by GE Healthcare (1 mentions)
Superdex 200 increase 10 300 gl column, by GE Healthcare (1 mentions)
2 protocols using protein g agarose resin
1
Estrone Sulfate Membrane Protein Assay
2
Monoclonal Antibody Production in FreeStyle 293F Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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KZ52 monoclonal antibody was produced via the transfection of plasmids encoding heavy and light chains into FreeStyle 293F cells using polyethylenimine. The day after transfection, cell cultures were supplemented with fresh media and with valproic acid to a final concentration of 2.2 mM. At 6 days post-transfection, supernatant was collected by spin centrifugation and filtration. IgG was isolated using Protein G agarose resin (Thermo Scientific) and subsequently purified via size exchange chromatography using a Superdex 200 Increase 10/300 GL column (GE Healthcare) on an AKTA Pure Protein Purification System (GE Healthcare).
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