Anti thy1.1 19e12

Arthritis was induced by either a single injection of 20 mg mannan (Sigma-Aldrich) intraperitoneally (Hashimoto et al., 2010 (link)), or adoptive transfer of CD4⁺ T cells intravenously (i.v.) (Hirota et al., 2007a (link)). CD4⁺ T cells were sorted from the spleen and peripheral LNs of SKG mice using MACS CD4 microbeads and LS column (Miltenyi Biotec) according to the manufacturer’s instruction. For depletion of ILCs, purified anti-Thy1.1 (19E12; Bio X cell), anti-Thy1.2 (30H12; Bio X Cell) or isotype control Rat IgG2b (LTF-2; Bio X Cell) antibody was i.v. injected. Joint swelling was scored as follows: 0, no joint swelling; 0.1, swelling of one finger joint; 0.5, mild swelling of wrist or ankle; 1.0, severe swelling of wrist or ankle. Scores for all fingers of forepaws and hindpaws, wrists and ankles were totaled for each mouse.