Brain sections were stained for GAD67, Parvalbumin (PV), and NeuN. Sections were imaged on a Zeiss Z2 fluorescent microscope with Apotome using a 20X/0.8 NA Plan-Apochromat air objective. A tiled field was imaged containing S1 somatosensory cortex L1-L6. Images were analyzed in FIJI by manual drawing of ROI containing each cortical layer and counting GAD67+ or PV+ soma. Density was calculated by dividing number of cells counted over the area of the ROI. All soma were confirmed to be NeuN+. Two images from two brain sections were averaged per animal, from 2 experiments; littermates were used within independent experiments.
Z2 fluorescent microscope
Manufactured by Zeiss
The Z2 fluorescent microscope is a sophisticated imaging instrument designed for high-performance fluorescence microscopy. It features advanced optics and illumination systems to enable detailed observation and analysis of fluorescently labeled samples. The core function of the Z2 is to provide researchers and scientists with a reliable and versatile tool for imaging and studying fluorescent specimens, such as cells, tissues, and other biological samples.
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Lab products found in correlation
2 protocols using z2 fluorescent microscope
1
Quantifying Cortical Interneuron Populations
2
Vibratome Sectioning and Immunostaining
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Vibratome sectioning of WISH analysed larvae (n = 3/stage or treatment) was carried out as previously described21 (link). Floating section immunohistochemistry against osteonectin and PCNA was carried out simultaneous using a 1/5000 dilution of rabbit saOSN polyclonal antisera and a 1/200 dilution of mouse monoclonal serum against PCNA (Dako). Detection was carried out simultaneously for both antiserums using respectively, goat-anti rabbit secondary antiserum conjugated with Hilyte-488 (1/400; Anaspec) and goat anti-mouse secondary antiserum conjugated with Hilyte-594 (1/400; Anaspec). Sections were placed on glass slides and Z-stacked images taken using a Zeiss Z2 fluorescent microscope.
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