Ultrapure (type 1) water obtained from Simplicity® Water Purification System (Millipore Corporation, Milford) was used for the preparation of all reagent solutions, buffers, and sample preparations. High purity analytical enzyme preparations, including pepsin (P7125, from porcine gastric mucosa), pancreatin (P1750, 4 × USP, porcine pancreas), bile (B8631, extract porcine), as well as the tubular cellulose membrane for dialysis, were purchased from Sigma-Aldrich Co. LLC. Electrophoresis purity reagents including Acrylamide, Sodium dodecyl sulfate (SDS), 2-Mercaptoethanol, Bromophenol Blue, Bis (N,N'-Methylen–bis-Acrylamide), Coomassie Brilliant Blue G-250 protein stain powder, N,N,N'N'-Tetra-methylenediamine, and Tris bas were supplied by BIO-RAD (Bio-Rad Laboratories, Inc.). All the experimental analyzes were carried out in triplicate.
Pepsin p7125
Manufactured by Merck Group
Sourced in United States
Pepsin (P7125) is a proteolytic enzyme derived from porcine gastric mucosa. It has a core function of hydrolyzing peptide bonds in proteins, particularly those involving the carboxyl group of aromatic amino acids such as phenylalanine, tryptophan, and tyrosine.
Automatically generated - may contain errors
Lab products found in correlation
Bromophenol blue, by Bio-Rad (1 mentions)
2 mercaptoethanol, by Bio-Rad (1 mentions)
Acrylamide, by Bio-Rad (1 mentions)
Coomassie brilliant blue g 250 protein stain powder, by Bio-Rad (1 mentions)
Bile b8631, by Merck Group (1 mentions)
Simplicity water purification system, by Merck Group (1 mentions)
Zein from maize z3625, by Merck Group (1 mentions)
Peg400, by Merck Group (1 mentions)
2 protocols using pepsin p7125
1
Purity Analysis of Enzymatic Reagents
2
Sustained-Release Diclofenac Formulation for Arthritis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Zein from maize (Z3625) was obtained from Sigma-Aldrich (Germany) and used as received.
This product was plasticized with polyethylene glycol 400, PEG 400, obtained from Merck-Schuchardt (Germany). The model drug, diclofenac sodium was obtained from Aarti drugs Ltd. (India) and was used as received. Diclofenac sodium was chosen on the basis that an extended release formulation of this, some are already available in market, can prove useful in management of signs and symptoms of patients with osteoarthritis and rheumatoid arthritis. Pepsin (p7125) and pancreatin (p8096) enzymes were purchased from Sigma-Aldrich (USA). Other materials used for the preparation of the dissolution media were reagents of standard grades and were supplied by AZ chem and Alpha Chemika (India).
This product was plasticized with polyethylene glycol 400, PEG 400, obtained from Merck-Schuchardt (Germany). The model drug, diclofenac sodium was obtained from Aarti drugs Ltd. (India) and was used as received. Diclofenac sodium was chosen on the basis that an extended release formulation of this, some are already available in market, can prove useful in management of signs and symptoms of patients with osteoarthritis and rheumatoid arthritis. Pepsin (p7125) and pancreatin (p8096) enzymes were purchased from Sigma-Aldrich (USA). Other materials used for the preparation of the dissolution media were reagents of standard grades and were supplied by AZ chem and Alpha Chemika (India).
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