Elix 5 milli q water purification system

Coix seed oil was extracted from Coix Lacryma-jobi by our group (Qu et al., 2014 ), the purity was determined as 87.6% using glycerin trilaurate as a reference. G-Rh2 (purity >98%) was purchased from Nanjing Zelang BioTECH Co. (Jiangsu, China). Etoposide (purity >99.9%) was bought from Sigma-Aldrich Co., Ltd. (Poole, UK). Cremophor^® RH40 was provided by BASF Co., Ltd. (Ludwigshafen, Germany). PEG400, amiloride, genistein, chlorpromazine, and ammonia chloride were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Roswell park memorial institute (RPMI) 1640 medium, fetal bovine serum (FBS), penicillin–streptomycin solution, MTT cell proliferation and cytotoxicity detection kit were bought from KeyGEN BioTECH Co., Ltd. (Jiangsu, China). The fluorescence probes including DiI and fluorescein isothiocyanate (FITC) were purchased from Beyotime Biotech Co., Ltd. (Shanghai, China). The water used in this study was produced by Elix^®5 Milli Q-water purification system (Millipore, Billerica, MA). The other chemical reagents were of analytical grade, unless otherwise statement.

Celastrol (purity >99.9%) was purchased from Sinopharma group Co., Ltd. (Shanghai, China). G Rh2 (purity >98%) was purchased from Nangjing zelang biotech Co., Ltd. (Jiangsu, China). Bis(4-nitrophenylcarbonate) polyethylene glycol (5 kDa, pNP-PEG-pNP) was purchased from Xiamen SINOPEG BioTech Co. (Xiamen, China). Pyrene was purchased from Invitrogen Co. (Carlsbad, CA). Coumarin 6 was provided by Sigma Co. (UK). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was obtained from Aladdin Chemical Co. (Shanghai, China). RPMI-1640 medium, penicillin–streptomycin solution and fetal bovine serum (FBS) were provided by Thermo Fisher Scientific Inc. (Beijing, China). Water in this study was prepared using Elix®5 Milli Q-water purification system (Millipore, MA). All other chemicals and reagents were analytical grade.