Uv 3310 spectrometer

All chemicals were commercially purchased from Sigma-Aldrich and used directly without further purification. Compound 1 (N-ethyl-4methylquinolinium iodide, MQ) was prepared according to the literature [29] . 1 H NMR (400 MHz) and 13 C NMR (100 MHz) spectra were measured on a Bruker AV spectrometer. High resolution mass spectrometry (HRMS) was obtained on HP-1100 LC-MS spectrometer. UV-Vis absorption spectra were performed on a Hitachi UV-3310 spectrometer. Fluorescence spectra were obtained with a FL-4500 fluorescence spectrometer. The cells imaging experiments were taken under a Nikon A1 confocal laser-scanning microscope with a 100 × objective lens.

A Bruker AV-400 spectrometer was used to record the 1 H NMR and 13 C NMR spectra. High-resolution mass spectra were measured on a HP-1100 LC-MS spectrometer. A Hitachi FL 4500 fluorometer and a Hitachi UV 3310 spectrometer were employed to measure the fluorescence spectra and UV-Vis spectra, respectively. A Nikon A1 confocal laserscanning microscope with a 100× objective lens was employed to acquire fluorescence images. The chromatography system consisted of a LC-10ATVP pump and SPD-10AVP UV-Vis detector (Shimadzu, Kyoto, Japan) with an injector (10 μL sample loop). The analysis was performed on an Optima Pak C 18 column (5 μm, 150 × 4.6 mm, RS tech Corporation, Daejeon, Korea) and Chromatography Data System N2000 (Surwit Technology, Hangzhou, China).

A Bruker DPX 400 NMR spectrometer was employed to determine the 1 H and 13 C NMR spectra using tetramethylsilane (TMS) as an internal standard. High-resolution mass spectra (HR-MS) were obtained with a HP-1100 LC-MS spectrometer. UV-vis absorption and fluorescence spectra were measured with a Hitachi UV-3310 spectrometer and a Hitachi FL-4500 fluorometer, respectively.