THP-1 and A2780 cells were seeded at the density of 2×105 cells/well and cultured in a 6-well plate. After washing, the cells were fixed in 2.5% buffered glutaraldehyde in the culture plate for 20 min at room temperature, scraped, centrifuged for pellet recovery, and stored overnight at 4°C. After post-fixation in 1% buffered osmium tetroxide for 2 h at 4°C, samples were rinsed, dehydrated in increasing concentrations of ethanol, embedded in epoxy resin and sectioned by means of ultramicrotome. Thick sections (80 nm) were stained with uranyl acetate followed by lead citrate and examined in a Philips CM100 (FEI Company, ThermoFisher, Waltham, MA, USA) Transmission Electron Microscope. Images were taken with an Olympus Megaview II digital camera integrated with iTEM image processing software.
Megaview 2 digital camera
Manufactured by Olympus
The Megaview II digital camera is a high-resolution imaging device designed for scientific and industrial applications. It features a large image sensor, advanced optics, and intuitive software controls to capture detailed digital images with precision.
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Lab products found in correlation
2 protocols using megaview 2 digital camera
1
TEM Imaging of THP-1 and A2780 Cells
2
Monocyte Adhesion to Endothelial Cells
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THP-1 monocytes were labelled with 5 μM calcein-AM (C3100MP; Molecular Probes, Oregon, USA) for 30 min and then seeded (2.5 × 105 cells) over hECs previously exposed to Tyr or its metabolites (100 μM) for 16 h and to TNF-α (10 ng mL -1 ) for additional 6 h. After the co-culture, the cells were washed with PBS and fluorescence was measured at excitation and emission wavelengths of 485 nm and 530 nm, respectively, using a Fluoroskan Microplate Fluorometer (ThermoFisher Scientific). THP-1 cells adhered to hECs were visualized by fluorescence microscopy with a motorized inverted fluorescence microscope IX81 equipped with a FITC filter and a Megaview-II digital camera (Olympus).
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