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Umi second strand synthesis module for quantseq fwd

Manufactured by Lexogen
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Sourced in Greenland

The UMI Second Strand Synthesis Module for QuantSeq FWD is a laboratory equipment product designed to perform second strand synthesis for the QuantSeq FWD library preparation workflow. It provides the necessary components and protocols to generate double-stranded cDNA from single-stranded cDNA templates.

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Lab products found in correlation

Strand ngs, by Agilent Technologies (2 mentions) Quantseq 3 mrna seq library prep kit fwd, by Illumina (2 mentions) Nexseq 500, by Illumina (1 mentions) Nextseq 500, by Illumina (1 mentions)

3 protocols using umi second strand synthesis module for quantseq fwd

1

Bulk RNA-Seq of Mouse Transcripts

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Total RNA was purified with a Trizol reagent. RNA-seq libraries were prepared using a QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina and UMI Second Strand Synthesis Module for QuantSeq FWD (Lexogen). Sequencing was performed on an Illumina NexSeq500 (Illumina) in a 75-base single-end mode. Obtained reads were mapped on mm10 genome, and UMI counts were measured using Strand NGS (Agilent). Expression data were normalized, and differentially expressed genes were identified with the EdgeR.
Shoda J., Tanaka S., Etori K., Hattori K., Kasuya T., Ikeda K., Maezawa Y., Suto A., Suzuki K., Nakamura J., Maezawa Y., Takemoto M., Betsholtz C., Yokote K., Ohtori S., & Nakajima H. (2022). Semaphorin 3G exacerbates joint inflammation through the accumulation and proliferation of macrophages in the synovium.
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2

Transcriptome Analysis by RNA-seq

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Total RNA was purified using the TRIzol reagent. RNA-seq libraries were prepared using a QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina and UMI Second Strand Synthesis Module for QuantSeq FWD (Lexogen). Sequencing was performed on an Illumina NexSeq500 (Illumina) in 75-base single-end mode. The obtained reads were mapped to the mm10 genome and UMI counts were measured using Strand NGS (Agilent). Expression data were normalized, and differentially expressed genes were identified using the EdgeR software.
Shoda J., Tanaka S., Etori K., Hattori K., Kasuya T., Ikeda K., Maezawa Y., Suto A., Suzuki K., Nakamura J., Maezawa Y., Takemoto M., Betsholtz C., Yokote K., Ohtori S, & Nakajima H. (2022). Semaphorin 3G exacerbates joint inflammation through the accumulation and proliferation of macrophages in the synovium. Arthritis Research & Therapy, 24, 134.
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3

QuantSeq 3' mRNA-Seq Library Prep for HNSCC

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Libraries were prepared using the QuantSeq 3’ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen, Inc., Greenland, NH), following the manufacturer’s instructions with the protocol alterations noted as follows. RNA input into library preparation was 40 ng for all samples. UMI Second Strand Synthesis Module for QuantSeq FWD (Lexogen, Inc., Greenland, NH) replaced Second Strand Synthesis Mix 1 in the workflow. All samples were processed with an OncoPrism-HNSCC Positive Control, OncoPrism-HNSCC Negative Control, and a No Template Control. The Positive (high scoring) and Negative (low scoring) controls were RNA extracted from RM-HNSCC samples as described above. Final libraries were sequenced to a minimum depth of 10 million single-end 75 base pair reads on a NextSeq500 (Illumina, San Diego, CA), following the manufacturer’s protocols.
Flanagan K.C., Earls J., Schillebeeckx I., Hiken J., Wellinghoff R.L., LaFranzo N.A., Bradley Z.S., Babbitt J., Westra W.H., Hsu R., Nadauld L., Mcleod H., Firth S.D., Sharp B., Fuller J., Vavinskaya V., Sutton L., Deichaite I., Bailey S.D., Sandulache V.C., Rendo M.J., Macdonald O.K., Welaya K., Wade JL I.I.I., Pippas A.W., Slim J., Bank B., Saccaro S.J., Sui X., Akhtar A., Balaraman S., Kossman S.E., Sonnier S.A., Shenkenberg T.D., Alexander W.L., Price K.A., Bane C.L., Ley J., Messina D.N., Glasscock J.I., Cohen E.E., Adkins D.R, & Duncavage E.J. (2023). Multidimensional biomarker predicts disease control in response to immunotherapy in recurrent or metastatic head and neck squamous-cell carcinoma. Journal of Cancer Research and Clinical Oncology, 149(15), 14125-14136.
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