Kyse 180

The human ESCC cell lines (TE-1D, KYSE-180, KYSE-520, ECA-109) were purchased from Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China). The use of 293T cells gifted from Dr. Kang was approved by the research ethics committee of The Third Hospital, Nanchang university. All the cells were cultured in DMEM medium containing 10% fetal bovine serum (FBS) in the incubator with 5% CO₂ at 37°C.

Human ESCC cell lines Eca109, Kyse180, Kyse140, Kyse410, Kyse510, Kyse520, TE-1,
and Kyse30 were obtained from Shanghai Institute of Biochemistry and Cell
Biology (Shanghai, China) and were cultured in Eagle’s Minimum Essential Medium
(Gibco) supplemented with 10% fetal bovine serum (FBS; Sigma). A normal human
esophageal epithelial cell (Het-1A) was purchased from Cell Bank of Shanghai
Institutes for Biological Sciences (Chinese Academy of Sciences) and then
cultured in RPMI-1640 medium with 10% FBS. All cells were cultured at a
humidified incubator at 37 °C in an atmosphere of 5% CO₂ and 95% air.
MiR-527 mimic, miR-527 inhibitor (miR-527-in), and negative control miRNAs were
purchased from GeneCopoeia Co Ltd.