Mcytomag 70 k 3 mouse cytokine chemokine magnetic bead panel

Blood sample was collected from the abdominal vena cava and the plasma supernatants were collected for further study. The levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), triglycerides (TG), and high-density lipoprotein cholesterol (HDL-C) were detected using an AU-2700 automatic biochemical analyzer (Olympus, Tokyo, Japan). The serum cytokine levels, including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), were detected using a Mcytomag-70 K-3 Mouse Cytokine/Chemokine Magnetic Bead Panel (Merck Millipore Co. LTD).

Mice were fasted overnight and euthanized. Blood was collected by removalling eyeballs and centrifuged by 1000× g for 10 min. Then supernatants were collected as the plasma samples for further detection. The levels of serous lipid including total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL) cholesterol and low-density lipoprotein (LDL) cholesterol were detected by an AU-2700 automatic biochemical analyzer (Olympus, Tokyo, Japan). The detection kits were purchased from Kang Taike Medical Co., Ltd. (Beijing, China), while the standard serous sample was supplied by Randox Laboratories Ltd. (Northern Ireland, UK).
The serous levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) were determined by Mcytomag-70K-3, Mouse Cytokine/Chemokine Magnetic Bead Panel (Merck Millipore Co., Ltd., Billerica, MA, USA) according to the manufacturer’s protocol.