Breast cancer cell lines were obtained from American Type Culture Collection (ATCC) and HepG2 from LGC Standards GmbH and cultured at 37°C, 5% CO2 in a humidified atmosphere. Cell line identity was authenticated by STR profiling, and all cells were verified to be mycoplasma‐free (Eurofins Genomics). HepG2 (RRID: CVCL_0027) and MCF7 (RRID: CVCL_0031) were cultured in Dulbecco's Modified Eagle's Medium (DMEM) High Glucose with 10% fetal bovine serum (FBS), both from Cytiva/HyClone. Insulin (Thermo Fisher Scientific) was added to 10 μg/mL for MCF7. BT‐474 (CVCL_0179) and T47D (CVCL_0553) were cultured in RPMI‐1640 (Cytiva/HyClone) with 10% FBS (Cytiva/HyClone). For experiments involving stimulation with ER ligands, cells were seeded in phenol red‐free DMEM (Cytiva/HyClone) with 2.5% charcoal‐stripped, dextran‐treated FBS (CSS‐FBS, Cytiva/HyClone).
Css fbs
Manufactured by Cytiva
The CSS-FBS is a laboratory equipment product designed for cell culture applications. It provides a controlled and consistent environment for the growth and maintenance of cells in vitro. The core function of the CSS-FBS is to enable the stable and reliable culture of cells, supporting their proliferation and viability.
Automatically generated - may contain errors
Lab products found in correlation
Insulin, by Thermo Fisher Scientific (2 mentions)
Mcf 7, by Thermo Fisher Scientific (2 mentions)
Mycoplasma free, by Eurofins (2 mentions)
Fetal bovine serum (fbs), by Cytiva (2 mentions)
Rpmi 1640, by Cytiva (2 mentions)
Hepg2, by LGC (2 mentions)
Hepg2, by Cytiva (2 mentions)
Dmem high glucose medium, by Cytiva (2 mentions)
Bt 474, by Cytiva (2 mentions)
2 protocols using css fbs
1
Breast and Liver Cancer Cell Culture
2
Breast and Liver Cancer Cell Culture
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Breast cancer cell lines were obtained from American Type Culture Collection (ATCC) and HepG2 from LGC Standards GmbH and cultured at 37°C, 5% CO2 in a humidified atmosphere. Cell line identity was authenticated by STR profiling, and all cells were verified to be mycoplasma‐free (Eurofins Genomics). HepG2 (RRID: CVCL_0027) and MCF7 (RRID: CVCL_0031) were cultured in Dulbecco's Modified Eagle's Medium (DMEM) High Glucose with 10% fetal bovine serum (FBS), both from Cytiva/HyClone. Insulin (Thermo Fisher Scientific) was added to 10 μg/mL for MCF7. BT‐474 (CVCL_0179) and T47D (CVCL_0553) were cultured in RPMI‐1640 (Cytiva/HyClone) with 10% FBS (Cytiva/HyClone). For experiments involving stimulation with ER ligands, cells were seeded in phenol red‐free DMEM (Cytiva/HyClone) with 2.5% charcoal‐stripped, dextran‐treated FBS (CSS‐FBS, Cytiva/HyClone).
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