Milliplex map high sensitivity t cell magnetic bead panel

MILLIPLEX MAP High Sensitivity T Cell Magnetic Bead Panel (Merck EMD Millipore, Billerica, MA, United States) for monocyte chemotactic protein-1 (MCP-1), monokine induced by IFN-γ (MIG), interferon-gamma (IFN-γ), interleukin (IL)-8, and interferon-inducible protein-10 (IP-10) was used according to the manufacturer's instructions. Luminex liquid suspension chip detection was performed using Huaying Biotechnologies (Shanghai, China). Briefly, tear samples were incubated in microbead-embedded 96-well plates overnight at 4°C, and subsequently incubated with detection antibody for 1 h at room temperature at next day. Next, streptavidin-phycoerythrin was added into each well of the plate and incubated for 30 min at room temperature, and the values were detected by a Luminex 200 system (Luminex Corporation, Austin, TX, United States).

Luminex liquid suspension chip detection was performed using Wayen Biotechnologies (Shanghai, China). MILLIPLEX MAP High Sensitivity T Cell Magnetic Bead Panel (Merck EMD Millipore, Billerica, MA, United States) for interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor alpha (TNF-α), and interferon-gamma (IFN-γ) was used according to the manufacturer's instructions. Briefly, tear samples were incubated in microbead-embedded 96-well plates overnight at 4°C and subsequently incubated with detection antibody for 1 h at room temperature. Next, streptavidin-phycoerythrin was added into each well of the plate and incubated for 30 min at room temperature, and the values were read using a Luminex 200 system (Luminex Corporation, Austin, TX, United States).

MILLIPLEX MAP High Sensitivity T Cell Magnetic Bead Panel (Merck EMD Millipore, Billerica, MA, United States) for monokine induced by IFN-γ (MIG), interferon-gamma (IFN-γ), interleukin (IL)-8, interferon-inducible protein-10 (IP-10), and monocyte chemotactic protein-1 (MCP-1) was used according to the manufacturer's instructions. Luminex liquid suspension chip detection was performed using Huaying Biotechnologies (Shanghai, China). Briefly, meibum samples were incubated in microbead-embedded 96-well plates overnight at 4°C and subsequently incubated with the detection antibody for 1 h at room temperature at the next day. Next, Streptavidin-Phycoerythrin was added into each well of the plate and incubated for 30 min at room temperature, and the values were detected by a Luminex 200 system (Luminex Corporation, Austin, TX, United States).