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Chloroform

Manufactured by Dingguo
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Sourced in China, Japan

Chloroform is a colorless, volatile liquid with a sweet, ethereal odor. It is commonly used in various laboratory applications as a solvent for organic compounds.

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Lab products found in correlation

Proteinase k, by Dingguo (2 mentions) Lysozyme, by Dingguo (2 mentions) Te buffer, by Dingguo (2 mentions) Tris saturated phenol chloroform isoamyl alcohol, by Dingguo (1 mentions) Chloroform isoamyl alcohol, by Dingguo (1 mentions) Trypsin, by Dingguo (1 mentions) Fetal bovine serum (fbs), by Dingguo (1 mentions) Ethanol, by Dingguo (1 mentions) Formaldehyde, by Dingguo (1 mentions) Rpmi 1640 medium, by Dingguo (1 mentions) El transfection reagent, by Transgene (1 mentions) Western blot reagents, by Beyotime (1 mentions) Trizol kit, by Takara Bio (1 mentions) Total rna extraction kit, by Tiangen Biotech (1 mentions) Sw620, by Procell (1 mentions) Phosphate buffered solution, by Dingguo (1 mentions)

4 protocols using chloroform

1

DNA Extraction with Phenol-Chloroform

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Tris-saturated phenol–chloroform-isoamyl alcohol (25:24:1), lysozyme, proteinase K, chloroform, isoamyl alcohol, acetone, and TE buffer were purchased from Beijing Dingguo Biotechnology Co. Ltd. 10% sodium dodecyl sulfate (SDS), 0.1 mol/L phosphaTE buffer solution (PBS) buffer, 5 mol/L NaCl, chloroform-isoamyl alcohol (24:1), cetyl trimethyl ammonium bromide (CTAB)/NaCl, 3 mol/L sodium acetate and 70% anhydrous ethanol, were prepared in the laboratory.
Zhou K., Peng M., Deng N., Tan Z, & Xiao N. (2022). Lactase bacteria in intestinal mucosa are associated with diarrhea caused by high-fat and high-protein diet. BMC Microbiology, 22, 226.
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2

Paclitaxel Delivery Using Silica Nanoparticles

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Paclitaxel (PAC) was supplied by Tianfeng Biotechnology Company (Xian, China). Tetrapropyl orthosilicate (TPOS), (3-aminopropyl)triethoxysilane (APTES), N-hydroxysuccinimide(NHS), and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) were purchased from Aladdin reagent company. Ethanol, NH3H2O, resorcinol, formaldehyde, hydrofluoric acid, Al(NO3)3, hyaluronic acid (HA), methyl tert-butyl ether, chloroform, acetonitrile, fetal bovine serum (FBS), RPMI 1640 medium, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT), dimethyl sulfoxide (DMSO), propidium iodide (PI), Annexin V-FITC, and trypsin were bought from Beijing Dingguo Changsheng Biotech Co., Ltd. (Beijing, China).
Gao Y., Hu L., Liu Y., Xu X, & Wu C. (2019). Targeted Delivery of Paclitaxel in Liver Cancer Using Hyaluronic Acid Functionalized Mesoporous Hollow Alumina Nanoparticles. BioMed Research International, 2019, 2928507.
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3

Investigating lncRNA in Colon Cancer

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The human colon cancer cell line SW620 was obtained from Wuhan Procell Life Science & Technology Co., Ltd, and hBD-1 was purchased from Sino Biological, Inc. (China). The total RNA extraction kit was purchased from TIANGEN Biotech (Beijing) Co., Ltd. The Trizol kit was acquired from Takara Bio Inc. (Japan), and chloroform was purchased from Beijing Dingguo Changsheng Biotechnology Co., Ltd. EL Transfection Reagent was purchased from TransGen Biotech Co., Ltd (Beijing). Additionally, si-lncRNA (Suzhou Gemma Biotechnology Co., Ltd.), BCA kit (Shanghai Epizyme Biotech Co., Ltd.), and Western blot reagents (Beyotime Biotech. Inc., Shanghai) were used. The antibody was purchased from Cell Signaling Technology Inc. (United States).
Zhao Y.X., Cui Y., Li X.H., Yang W.H., An S.X., Cui J.X., Zhang M.Y., Lu J.K., Zhang X., Wang X.M., Bao L.L, & Zhao P.W. (2024). Human β-defensin-1 affects the mammalian target of rapamycin pathway and autophagy in colon cancer cells through long non-coding RNA TCONS_00014506. World Journal of Gastrointestinal Oncology, 16(4), 1465-1478.
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4

Nucleic Acid Extraction Protocol

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Proteinase K, lysozyme, Tris-saturated phenol: chloroform: isoamyl alcohol (25:24:1), TE buffer, and acetone were purchased from Beijing Dingguo Biotechnology Co. Ltd. About 0.1 mol/L phosphate-buffered solution (PBS) buffer, TE buffer, 10% sodium dodecyl sulfate (SDS), 5 mol/L NaCl, hexadecyl trimethyl ammonium bromide (CTAB)/NaCl, chloroform: isoamyl alcohol (24:1), 3 mol/L sodium acetate and 70% anhydrous ethanol, were prepared in the laboratory.
Zhou K., Peng M., Tan Z, & Xiao N. (2023). Diarrhea Caused by High-Fat and High-Protein Diet Was Associated With Intestinal Lactase-Producing Bacteria. The Turkish Journal of Gastroenterology, 34(7), 691-699.
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