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Actin sc 8432

Manufactured by Santa Cruz Biotechnology
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Actin (sc-8432) is an antibody product offered by Santa Cruz Biotechnology. It is a protein that plays a fundamental role in various cellular processes, including cell structure, motility, and division. The description of this product is limited to its core function as an actin antibody, without any interpretation or extrapolation on its intended use.

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Lab products found in correlation

Z vrpr fmk, by Enzo Life Sciences (2 mentions) Phosphorylation specific antibodies to erk1 2 9101, by Cell Signaling Technology (2 mentions) Iκbα 9246, by Cell Signaling Technology (2 mentions) Dna oligo probes for nf κb e3291, by Promega (2 mentions) Oct 1 e3241, by Promega (2 mentions) Pma 16561 29 8, by Thermo Fisher Scientific (2 mentions) Ionomycin 56092 82 1, by Thermo Fisher Scientific (2 mentions) Il 6 neutralizing antibody, by Abcam (2 mentions) Tnf α, by Thermo Fisher Scientific (2 mentions) Recombinant human egf, by Merck Group (2 mentions) Recombinant mouse bmp4, by R&D Systems (1 mentions) Lamin b1 33 2000, by Thermo Fisher Scientific (1 mentions) Recombinant il 6, by R&D Systems (1 mentions) Chronolume, by Chrono-Log (1 mentions) Sodium cholate hydrate, by Merck Group (1 mentions) 4 hydroxynonenal 4 hne, by Merck Group (1 mentions) Proliferating cell nuclear antigen (pcna), by GeneTex (1 mentions) 2 4 dinitrophenylhydrazine, by Merck Group (1 mentions) Gtx100539, by GeneTex (1 mentions) D fructose, by Merck Group (1 mentions)

11 protocols using actin sc 8432

1

Immunoblotting for OXPHOS and Signaling

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Immunoblotting was performed essentially as described [3 (link)], with the following primary antibodies: total OXPHOS Antibody Cocktail (ab110413, Abcam); p IR/IGF beta (3021), p AKT (9271), AKT (9272), p38 MAPK (9212), p p38 MAPK (9211), HSL (4107), p SMAD1/5/8 (13820), and B tubulin (2128) (all from Cell Signaling Technology, Danvers, MA, USA); IRS1 (06–248, Upstate/Merck Millipore); IR beta (sc-711), p JNK (sc-6254), BMP4 (sc-73536), SMAD 1/5/8 (N-18)-R (sc 6031-R), GAPDH (sc-47724), and actin (sc-8432) (all from Santa Cruz Biotechnology, Dallas, TX, USA); and UCP1 (MAB6158, R&D Systems). Recombinant mouse BMP4 (R&D Systems) was used as a positive control where indicated. Quantifications were performed with normalization against loading controls.
Hoffmann J.M., Grünberg J.R., Hammarstedt A., Kroon T., Greiner T.U., Maurer S., Elias I., Palsdottir V., Bosch F., Boucher J., Hedjazifar S, & Smith U. (2019). BMP4 gene therapy enhances insulin sensitivity but not adipose tissue browning in obese mice. Molecular Metabolism, 32, 15-26.
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2

INPP5F Knockdown Using shRNAs

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Antibodies against to INPP5F (H00022876-B01P) were purchased from Novus Biologicals, Littleton, CO; Stat3 (sc-7179), p-Stat3 (sc-8059), GFP (sc-8334) and Actin (sc-8432) from Santa Cruz Biotechnology, Dallas, TX; V5 (A01724) from GenScript, Piscataway, NJ; Lamin B1 (33-2000) from Invitrogen (Life Technologies, Carlsbad, CA). Recombinant IL-6 was purchased from R&D System. The plasmids pLenti6/UbC/V5-DEST, pDEST47-DEST and ViraPower mix were purchased from Life Technologies; and INPP5F ShRNAs were from Open Biosystems (GE Dharmacon, Lafayette, CO).
Kim H.S., Li A., Ahn S., Song H, & Zhang W. (2014). Inositol Polyphosphate-5-Phosphatase F (INPP5F) inhibits STAT3 activity and suppresses gliomas tumorigenicity. Scientific Reports, 4, 7330.
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3

Platelet Agonists and Signaling Pathways

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Platelet agonists, including ADP, collagen, and thrombin as well as CHRONO-LUME® were purchased from Chrono-log (Havertown, PA). Collagen related peptide (CRP) was a gift from Dr. Peter Newman (Blood Research Institute, WI) as mentioned before.24 (link) Antibodies to phosphorylated AKT (4060S), pan-AKT (2920S), TYMP (4307S), and GST (2624S) were purchased from Cell Signaling Technology (Danvers, MA). Antibodies to TYMP (SC-56584), Lyn (SC-7274), and Actin (sc-8432) were purchased from Santa Cruz Biotechnology (Dallas, TX). Antibody to TYMP was also purchased from abcam (ab180783). FITC-conjugated P-selectin antibody (Cat#553744) and the isotype control IgG were purchased from BD Biosciences (San Jose, CA). Tipiracil hydrochloride (TPI) (T2366) was purchased from TargetMol (Boston, MA). All other chemical reagents were purchased from Sigma (St. Louis, MO) except where specifically indicated.
Belcher A., Zulfiker A.H., Li O.Q., Yue H., Gupta A.S, & Li W. (2020). Targeting Thymidine Phosphorylase with Tipiracil Hydrochloride Attenuates Thrombosis without Increasing Risk of Bleeding in Mice. Arteriosclerosis, thrombosis, and vascular biology, 41(2), 668-682.
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4

Cell Size Determination Protocol

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The cell size was determined as described with some modifications.79, 80 Briefly, the cells were fixed in 4% paraformaldehyde, followed by staining with either rhodamine‐conjugated wheat‐germ agglutinin (WGA; Life Technologies; 1:200 dilution) or actin (SC‐8432, Santa Cruz Biotechnology). The images were captured using a Zeiss confocal microscope (Model 710) and Image J was used to measure the area of the cells.
Gaddam R.R., Kim Y., Jacobs J.S., Yoon J., Li Q., Cai A., Shankaiahgari H., London B., Irani K, & Vikram A. (2022). The microRNA‐204‐5p inhibits APJ signalling and confers resistance to cardiac hypertrophy and dysfunction. Clinical and Translational Medicine, 12(1), e693.
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5

Evaluation of Kidney Injury Markers

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Sodium cholate hydrate, cholesterol, D-(+)-glucose, D-(−)-fructose, 2,4-dinitrophenylhydrazine (DNPH), and 4-hydroxynonenal (4HNE) were purchased from Sigma (St. Louis, MO, USA). Antibodies to nephrin (sc-376522), NGAL (sc-515876), IL-1β (sc-52012), IL-6 (sc-57315), TNF-α (sc-52746), and actin (sc-8432) were acquired from Santa Cruz Biotechnology, Inc., (Dallas, TX, USA). KIM-1 (GTX85067), Nrf2 (GTX103322), Keap1 (GTX60660), NFκB p65 (GTX102090), IκB (GTX82797), and PCNA (GTX100539) were from GeneTex (Irvine, CA, USA). Goat anti-rabbit and goat anti-mouse IgG-HRP were purchased from Cell Signaling Technology (Danvers, MA, USA). All other chemicals used were of the highest purity available.
Arellano Buendia A.S., Juárez Rojas J.G., García-Arroyo F., Aparicio Trejo O.E., Sánchez-Muñoz F., Argüello-García R., Sánchez-Lozada L.G., Bojalil R, & Osorio-Alonso H. (2023). Antioxidant and anti-inflammatory effects of allicin in the kidney of an experimental model of metabolic syndrome. PeerJ, 11, e16132.
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6

Western Blot Analysis of Signaling Proteins

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Cells were lysed with 200 µL of Ripa buffer containing proteinase inhibitor cocktails (Fisher Scientific, Pittsburgh, PA), sheared 10 times with a 28-gauge needle, spun at 16,000 × g for 30 min, normalized by protein concentration as determined by the Bradford method, and boiled for 5 min. Normalized lysate was resolved by 4%–12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Fisher Scientific, Pittsburgh, PA) and immunoblotting with indicated antibodies; p-EGFR (#3777S), p-STAT3 (#9134S), p-AKT (#4060S), EGFR (4267S), STAT3 (9139S), and AKT (9272S), which were all purchased from Cell Signaling Technology (Danvers, MA). Actin (SC-8432) was purchased from the Santa Cruz Biotechnology (Dallas, TX)
Pan J., Lee Y., Zhang Q., Xiong D., Wan T.C., Wang Y, & You M. (2016). Honokiol Decreases Lung Cancer Metastasis through Inhibition of the STAT3 Signaling Pathway. Cancer prevention research (Philadelphia, Pa.), 10(2), 133-141.
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7

Signaling Pathway Activation Analysis

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Phosphorylation-specific antibodies to ERK1/2 (9101) and IκBα (9246) were purchased from Cell Signaling Technology (Danvers, MA). Antibodies against phosphorylated EGFR (sc-12351), IκBα (sc-371), lamin B (sc-6216), ERK (sc-154), IKKγ (FL-419) and actin (sc-8432) were purchased from Santa Cruz Biotechnology (Dallas, TX). Monoclonal antibodies against the C terminus of MALT1 were generated in the Genentech (South San Francisco, CA) central production facility. DNA-oligo probes for NF-κB (E3291) and Oct-1 (E3241) were purchased from Promega (Madison, WI). Recombinant human EGF was purchased from Sigma-Aldrich (St. Louis, MO). PMA (16561-29-8) and ionomycin (56092-82-1) were purchased from Fisher Scientific (Pittsburgh, PA). IL-6 neutralizing antibodies were purchased from Abcam (ab6672) and used at 1:400 dilution. TNFα was purchased from Thermo Fisher Scientific (Rockford, IL). MALT1 protease inhibitor z-VRPR-Fmk was purchased from Enzo Life Sciences, Inc. (Farmingdale, NY).
Pan D., Jiang C., Ma Z., Blonska M., You M.J, & Lin X. (2015). MALT1 is required for EGFR induced NF-κB activation and contributes to EGFR-driven lung cancer progression. Oncogene, 35(7), 919-928.
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8

Signaling Pathway Activation Analysis

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Phosphorylation-specific antibodies to ERK1/2 (9101) and IκBα (9246) were purchased from Cell Signaling Technology (Danvers, MA). Antibodies against phosphorylated EGFR (sc-12351), IκBα (sc-371), lamin B (sc-6216), ERK (sc-154), IKKγ (FL-419) and actin (sc-8432) were purchased from Santa Cruz Biotechnology (Dallas, TX). Monoclonal antibodies against the C terminus of MALT1 were generated in the Genentech (South San Francisco, CA) central production facility. DNA-oligo probes for NF-κB (E3291) and Oct-1 (E3241) were purchased from Promega (Madison, WI). Recombinant human EGF was purchased from Sigma-Aldrich (St. Louis, MO). PMA (16561-29-8) and ionomycin (56092-82-1) were purchased from Fisher Scientific (Pittsburgh, PA). IL-6 neutralizing antibodies were purchased from Abcam (ab6672) and used at 1:400 dilution. TNFα was purchased from Thermo Fisher Scientific (Rockford, IL). MALT1 protease inhibitor z-VRPR-Fmk was purchased from Enzo Life Sciences, Inc. (Farmingdale, NY).
Pan D., Jiang C., Ma Z., Blonska M., You M.J, & Lin X. (2015). MALT1 is required for EGFR induced NF-κB activation and contributes to EGFR-driven lung cancer progression. Oncogene, 35(7), 919-928.
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9

Immunoprecipitation and Western Blot Analysis

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Cell lysates from BMDMs were immunoprecipitated with indicated antibody-conjugated agarose. The resulting immunoprecipitates and lysates were subjected to SDS-PAGE and then blotted using indicated antibodies. Phosphorylated IκB kinase α/β (#2697), extracellular signal-regulated kinase 1/2 (#9101), P38 (#4631), IκBα (#9246), and STAT3 (##9145); cleaved caspase 3 (#9664); and P38 (#9212) were purchased from Cell Signaling Technology. Antibodies against IκB kinase α (sc-7218), IκBα (sc-371), Noxa (sc-30209), and actin (sc-8432) were purchased from Santa Cruz Biotechnology. An anti-CARD9 antibody was purchased from Sigma (C7862).
Wang T., Pan D., Zhou Z., You Y., Jiang C., Zhao X, & Lin X. (2016). Dectin-3 Deficiency Promotes Colitis Development due to Impaired Antifungal Innate Immune Responses in the Gut. PLoS Pathogens, 12(6), e1005662.
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10

Western Blot Analysis of Shp2 Ubiquitination

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Western blotting was performed as previously described. In brief, cell lysates with equal concentration of total proteins were separated by 10% SDS-polyacrylamide gel electrophoresis, transferred to polyvinylidene difluoride membranes and analyzed by immunoblotting with specific antibodies. Grayscale bands were quantified using Quantity One software (Bio-Rad). For ubiquitination assays, the Shp2-containing complex was first isolated from total cell lysates by immunoprecipitation and separated on SDS polyacrylamide gels. Ubiquitination of Shp2 was detected by western blotting with a specific antibody against ubiquitin (Santa Cruz Biotechnology, sc-9133, Santa Cruz, CA, USA). Antibodies against Shp2 (SH-PTP2, sc-280), PPARγ (sc-7196), β-actin (sc-8432) and ERK1 (sc-93) were purchased from Santa Cruz Biotechnology. Antibodies against p-ERK1/2 (4370 s), p-STAT3 (9145 s), STAT3 (9139 s) and GAPDH (2118 s) were obtained from Cell Signaling (Bererly, MA, USA).
Tao J., Zheng L., Meng M., Li Y, & Lu Z. (2016). Shp2 suppresses the adipogenic differentiation of preadipocyte 3T3-L1 cells at an early stage. Cell Death Discovery, 2, 16051-.
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