60 f254 aluminium plates

General procedures: Commercial reagents were used as received without further purification. Microwave irradiation reaction was performed with an InitiatorTM 2.0 device, Biotage. Reactions were followed with thin-layer chromatography (TLC) (using 0.20 mm silica or alumina gel 60 F₂₅₄ aluminium plates, Merck) and visualisation was achieved with UV light (254 and 365 nm). Purifications were achieved through recrystallization or flash chromatography (using 40–63 µM silica, Merck). ¹H and ¹³C NMR spectra were recorded on a Bruker AC 300 or 400 spectrometer by using DMSO-d₆ as the solvent and internal standard. Chemical shifts are reported in ppm and coupling constants (J) are given in Hertz. Spin multiplicities are reported as follows: s = singlet, d = doublet, dd = doublet of doublet, m = multiplet, q = quadruplet, quint = quintuplet, t = triplet. Melting points were measured on a Stuart SMP3 melting point apparatus and are uncorrected. IR spectra were obtained on Perkin-Elmer 1600 spectrophotometer. Elemental analyses were performed at the CNRS Analysis Laboratory, Gif-sur-Yvette, France.

The preliminary screening of the obtained polar extracts (final concentration 30 mg/mL) was performed with TLC (precoated silica gel 60 F254 aluminium plates, Merck, Milan, Italy) eluted with ethylacetate/formic acid/acetic acid/water (100:11:11:27 v/v). Visualization of compounds was obtained with Natural Product-Polyethylenglycol Reagent (NP/PEG, Sigma, Milan, Italy). After spraying, TLC plates were observed under UV-366 nm light.