Anti mouse fluorochrome conjugated monoclonal antibodies

Manufactured by BD

Anti-mouse fluorochrome-conjugated monoclonal antibodies are laboratory reagents used to detect and analyze specific mouse proteins or cell surface markers in various experimental applications. These antibodies are conjugated with fluorescent dyes, allowing for the visualization and quantification of the target molecules using flow cytometry, fluorescence microscopy, or other fluorescence-based techniques.

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Lab products found in correlation

Mitotracker red, by Thermo Fisher Scientific (3 mentions) Live dead fixable aqua dye, by Thermo Fisher Scientific (1 mentions) Cd16 32 2.4g2, by BD (1 mentions) Onecomp ebeads beads, by Thermo Fisher Scientific (1 mentions) Mitotracker deep red, by Thermo Fisher Scientific (1 mentions) Lsr 2, by BD (1 mentions)

Close spelling variants of this product

Mouse anti-human fluorochrome-conjugated monoclonal antibodies Fluorochrome-conjugated monoclonal antibodies Anti-mouse monoclonal antibodies Mouse monoclonal antibodies Anti-mouse antibodies Mouse anti

2 protocols using anti mouse fluorochrome conjugated monoclonal antibodies

Isolation and Identification of Mast Cells and Basophils

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Small intestinal lamina propria cells or cells from the skin were isolated as described previously. ^{27 (link),28 (link)} Single cell suspensions were incubated with Aqua Live/Dead Fixable Dye (Invitrogen) for dead cell exclusion and stained with anti-mouse fluorochrome-conjugated monoclonal antibodies against B220, CD3e, CD4, CD5, CD8, CD11c, NK1.1, CD19, IgE, CD45, CD49b or CD117 (c-kit) (BD Bioscience, BioLegend, eBioscience). Mast cells were identified as live, lin⁻ (CD3,CD5,CD19,CD11c,NK1.1), CD45⁺, c-kit⁺, IgE⁺ cells and basophils as live, lin⁻, CD45⁺, c-kit⁻, CD49b⁺, IgE⁺ cells.

Noti M., Kim B.S., Siracusa M.C., Rak G.D., Kubo M., Moghaddam A.E., Sattentau Q.A., Comeau M.R., Spergel J.M, & Artis D. (2014). Exposure to food allergens through inflamed skin promotes intestinal food allergy via the TSLP-basophil axis. The Journal of allergy and clinical immunology, 133(5), 1390-1399.e6.

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Macrophage Phenotyping by Flow Cytometry

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Thioglycollate-elicited peritoneal macrophages from control or PPARγ knockout mice were FC-blocked with CD16/32 (2.4G2, 1:50; BD Bioscience) and rat IgG (1:50; Fisher Scientific) prior to staining with anti-mouse fluorochrome-conjugated monoclonal antibodies (clone and concentration shown; BD Bioscience, BioLegend, eBioscience) specific for CD11b (M1/70; 1:500) and F4/80 (BM8; 1:250). Cells were also stained with 100 nM MitoTracker red or 750 nM MitoTracker deep red (Thermo Fisher). Compensation was performed with cells or OneComp eBeads beads (Thermo Fisher). Cells were analyzed with a BD LSR II running DiVa software (BD Bioscience) and analyzed with FlowJo software (version 10.4, Tree Star).

Nelson V.L., Nguyen H.C., Garcìa-Cañaveras J.C., Briggs E.R., Ho W.Y., DiSpirito J.R., Marinis J.M., Hill D.A, & Lazar M.A. (2018). PPARγ is a nexus controlling alternative activation of macrophages via glutamine metabolism. Genes & Development, 32(15-16), 1035-1044.

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