Methylseleninic acid

Myc-CaP, an androgen sensitive mouse prostate cancer cell line, is derived from c-Myc transgenic mouse. LNCaP (a human androgen sensitive prostate carcinoma cell line), DU145 and PC3 cell lines are purchased from American Type Culture Collection (ATCC). These cell lines were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin, and incubated in a humidified incubator with 5% CO₂ at 37 °C. Myc-CaP cells and DU145 cells stably expressing control shRNA (Sigma), INMT shRNA (Sigma) or SMYD3 shRNA (Sigma) were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and puromycin (10 μg/mL). Myc-CaP cells engineered to stably express control vector and pCMV-INMT were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and hygromycin (100 μg/mL). DMT was purchased from Cayman Chemical company, bis(7)-Tacrine, BCl-121, Methylseleninic acid (MSA), and Se-(Methyl)selenocysteine hydrochloride (MSC) were purchased from Sigma.