Malt extract

Manufactured by Solarbio

Sourced in China

Malt extract is a concentrated, syrupy substance derived from the germination of cereal grains, typically barley. It serves as a nutrient-rich medium for the cultivation of microorganisms in laboratory settings.

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Lab products found in correlation

Yeast extract, by Thermo Fisher Scientific (1 mentions) Peptone, by Thermo Fisher Scientific (1 mentions) Mgso4, by Solarbio (1 mentions) Yeast extract, by Solarbio (1 mentions) Peptone, by Solarbio (1 mentions) Peptone, by Maclin Biochemical Technology (1 mentions) Mgso4, by Maclin Biochemical Technology (1 mentions) Kh2po4, by Maclin Biochemical Technology (1 mentions) Urokinase, by Yuanye Bio-Technology (1 mentions)

2 protocols using malt extract

Microbial Growth Media Preparation

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Glucose medium (w/v): Glucose (Aladdin, Shanghai, China) 10%, peptone (Oxoid, UK) 2%, yeast extract (Oxoid, UK) 1%.
Sugarcane molasses medium: Sugarcane molasses (Zhongfuxin, Guangxi, China) 20 BX°, (NH₄)₂SO₄ (Macklin, Shanghai, China) 0.1%, KH₂PO₄ (Macklin, Shanghai, China) 0.1%, MgSO₄ (Macklin, Shanghai, China) 0.04% (w/v).
Nitrogen-rich medium (w/v): Soybean meal (Macklin, Shanghai, China) 5%, peptone 2%, yeast extract 1%.
YEPD medium (w/v): Glucose 2%, peptone 2%, yeast extract 1%.
GPYM medium (w/v): Glucose 4%, peptone 0.5%, yeast extract 0.5%, MgSO₄·7 H₂O 0.1%, malt extract (Solarbio, Beijing, China) 0.5%.
About 100 mL of Glucose medium, sugarcane molasses medium, and nitrogen-rich medium were prepared, respectively, in 250 mL conical flask, and 10% (v/v) yeast seed liquids were inoculated, respectively, and cultivated at 28°C, 150 rpm for 3 days.

Qu C., Peng L., Fei Y., Liang J., Bai W, & Liu G. (2023). Screening ester-producing yeasts to fortify the brewing of rice-flavor Baijiu for enhanced aromas. Bioengineered, 14(1), 2255423.

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Bacterial Strain Cultivation and Sourcing

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B. subtilis BSNK-5 was identified in our laboratory and stored at −80 °C in a refrigerator with 15% glycerol (w/v). S. lactis (BNCC 190729) and Micrococcus luteus (BNCC 102589) were purchased from BeNa Culture Collection. S. clavuligerus (ATCC 27064) was purchased from Shanghai Guyan Industrial Co., Ltd. (Shanghai, China). The yeast extract and tryptone were purchased from Oxoid (LP0021, LP0042B, Beijing, China). DeMan Rogosa and Sharpe (MRS) medium was purchased from AOBOX (02-293, Beijing, China). The Nutrient Broth (NB) medium was purchased from Qingdao Hope Bio-Technology Co., Ltd. (HB0108, Qingdao, China). The malt extract was purchased from Solarbio Science & Technology Co., Ltd. (M8130, Beijing, China).
Urokinase and nisin were purchased from Yuanye Bio-Technology Co., Ltd. (9039-53-6, 1414-45-5, Shanghai, China). CA was purchased from the National Institutes for Food and Drug Control (6SNT-6F3D, Beijing, China).
The beef extract (BE) was purchased from AOBOX (01-009, Beijing, China). SP was purchased from Solarbio Science & Technology Co., Ltd. (1008C052, Beijing, China). SMP, SPC, SPI, and SPH were supplied by Shandong Vandefu Co., Ltd. (Shandong, China).

Wen W., Hu M., Gao Y., Zhang P., Meng W., Zhang F., Fan B., Wang F, & Li S. (2024). Effect of Soy Protein Products on Growth and Metabolism of Bacillus subtilis, Streptococcus lactis, and Streptomyces clavuligerus. Foods, 13(10), 1525.

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