Sitostanol

Manufactured by Merck Group

Sourced in United States

Sitostanol is a plant-derived compound used in laboratory settings. It functions as a phytosterol, a type of naturally occurring compound found in plants. Sitostanol is commonly utilized in research and analysis applications, though its specific uses may vary depending on the research context.

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Lab products found in correlation

6 protocols using sitostanol

Sitosterol and Sitostanol Cell Exposure

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Stock solutions of sitosterol and sitostanol (Sigma) were prepared in ethanol and provided to the different in vitro cell models. Final sitosterol and sitostanol concentrations used were 12 µM and 1.2 µM, respectively, and compared to ethanol as carrier control. All conditions were set at identical ethanol concentration of 0.25%.

Nascimento E.B., Konings M., Schaart G., Groen A.K., Lütjohann D., van Marken Lichtenbelt W.D., Schrauwen P, & Plat J. (2019). In vitro effects of sitosterol and sitostanol on mitochondrial respiration in human brown adipocytes, myotubes and hepatocytes. European Journal of Nutrition, 59(5), 2039-2045.

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Sterol Extraction and Identification

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All reagents were purchased from Merck (Darmstadt, Germany). GLC-463 mix was from Nu-Check (Elysian, MN, USA), while FAME 189-19, (+)-α-tocopherol, 5α-cholestan-3β-ol, campesterol, campestanol, stigmasterol, β-sitosterol, sitostanol and Δ⁵-avenasterol were from Sigma-Aldrich (St. Louis, MO, USA).

Marzocchi S., Messia M.C., Marconi E., Caboni M.F, & Pasini F. (2023). Lipid Process Markers of Durum Wheat Debranning Fractions. Foods, 12(16), 3036.

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Flaxseed Extraction and Characterization

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Flaxseed was obtained from the Gansu Academy of Agricultural Sciences, China. The raw material was cleaned to remove dust, stone, other seeds, etc. The cleaned seed was dried at 50℃ until reducing to the moisture of approximately 6％. The dried sample was ground in a blender and then pass through a 30-mesh sieve. The ground seed was sealed and stored in the dark at 4℃ until use.
n-Butane solvent (99.5％) was obtained from Longyu Chemical Co., Ltd. (Puyang, China) . Fatty acids and phytosterols standards ( Campesterol, Stigmasterol, β-Sitosterol, Sitostanol and 5α-cholestanol) were purchased from Sigma-Aldrich Chemical Co., Ltd. (Shanghai, China) . All other chemicals used in study were at least of analytical grade.

Wang S., Zhang Z.S., Zhang T.F, & Wang X.D. (2020). Extraction and Characterization of Flaxseed Oil Obtained with Subcritical n-Butane. Journal of oleo science, 69(9).

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Quantification of Oryzanol and Sterols

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All solvents and chemicals were of analytical grade or HPLC/GC grade and purchased from RCI Labscan (Bangkok, Thailand). The total γ-oryzanol (98.5%) standard was purchased from Tsuno Rice Fine Chemical Co., Ltd. (Wakayama, Japan). PC standards, including docosanol (C₂₂), tetracosanol (C₂₄), hexacosanol (C₂₆), octacosanol (C₂₈) and triacontanol (C₃₀), and phytosterol standards, including campesterol, stigmasterol, β-sitosterol, sitostanol, and 5α-cholestane, were purchased from Sigma-Aldrich (St. Louis, MO, USA). N,O-bis(trimethylsilyl)-trifluoroacetamide (BSTFA) with 1% trimethylchlorosilane (TMCS) and pyrogallol were also purchased from Sigma-Aldrich (St. Louis, MO, USA).

Wongwaiwech D., Weerawatanakorn M, & Boonnoun P. (2020). Subcritical dimethyl ether extraction as a simple method to extract nutraceuticals from byproducts from rice bran oil manufacture. Scientific Reports, 10, 21007.

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Enzymatic Synthesis of Lipid Esters

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-sitostanol, palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid, and pnitrophenyl butyrate were purchased from Sigma-Aldrich (St. Louis, MO, USA). The enzymes NS 40020 (CalA) and Lipozyme® (CalB) were kindly provided by Novozymes. Other chemicals and solvents were of the purest available grade, provided by Sigma-Aldrich (St. Louis, MO, USA) or Merck (Darmstadt, Germany).

Hakalin N.L.S., Molina-Gutiérrez M., Prieto A, & Martínez M.J. (2018). Optimization of lipase-catalyzed synthesis of β-sitostanol esters by response surface methodology. Food chemistry, 261.

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Comprehensive Characterization of FMBS, FMPB, and FMMB

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FMSB, FMPB and FMMB (mixture of FMBS and FMPB) used in this research were supplied by the market of Qinshui Country (Shanxi Province, China) . Prior to analysis, all samples were dried, ground to powder, passed through a 60-mesh screen, and then stored in a refrigerator until analyzed.
Individual standards of fatty acid methyl esters (FAMEs, purity ≥ 99％) including methyl ester of lauric (12:0) , myristic (14:0) , palmitic (16:0) , palmitoleic (16:1) , stearic (18:0) , oleic (18:1) , linoleic (18:2) , linolenic (18:3) , arachidic (20:0) , eicosenoic (20:1) , behenic (22:0) , lignoceric (24:0) , 17 amino acids, 8 isoforms of tocopherols (Ts) and tocotrienols (T3s) , 5-α cholesterol standard (purity ≥ 95％) , campesterol, stigmasterol, β-sitosterol, sitostanol, squalene (purity ≥ 98％) , acetonitrile, n-hexane and methanol of chromatographic grade were all obtained from Sigma -Aldrich Co. (St. Louis, MO, USA) . Other reagents were of analytical pure grade and directly used without further purification.

Ji J., Liu Y., Ge Z., Zhang Y, & Wang X. (2019). Oleochemical Properties for Different Fractions of Foxtail Millet Bran. Journal of oleo science, 68(8).

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