Cyclind1 clone ep12

Manufactured by Agilent Technologies

The CyclinD1 clone EP12 is a monoclonal antibody that targets the protein cyclinD1. It is designed for use in immunohistochemistry and other applications that require the detection of cyclinD1 expression in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Clone EP12 (2 citations)

3 protocols using cyclind1 clone ep12

Immunocytochemical Analysis of Malignant Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunocytochemical analysis was possible only on CBs, on three micron-thick unstained sections. An initial panel of antibodies was used in malignant cases to differentiate lymphoma from carcinoma and sarcoma, namely pan-cytokeratin (clone AE1/3; DAKO, Carpinteria, CA, USA), CD45/LCA (Clones 2B11+PD7/26; DAKO), CD20 (clone L26; DAKO), CD79α (clone JCB 117; DAKO), CD3 (polyclonal; DAKO), EMA (clone E29; DAKO), CD30 (clone Ber-H2; DAKO), CD15 (clone Carb-3; DAKO) and PAX5 (clone DAK-PAX5; DAKO). In case a diagnosis of B-lineage NHL had been advanced, a second lineage of immunocytochemical markers was performed, including BCL2 (clone 124; DAKO), BCL6 (clone PG-B6p; DAKO), CD10 (clone 56C6; DAKO), CD5 (clone 4C7; DAKO), CyclinD1 (clone EP12; DAKO), MUM1/IRF4 (clone MUM1p; DAKO) and Mib1 (clone Ki67; DAKO). All staining procedures were carried out on Autostainer Link48 (DAKO, DAKO), according to the manufacturer’s instructions and in the presence of appropriate controls. Neither morphology nor first- nor second-line immunocytochemical analyses aroused suspicion of T-lineage NHL.

Parente P., Covelli C., Zanelli M., Trombetta D., Carosi I., Carbonelli C., Sperandeo M., Mastracci L., Biancofiore G., Zizzo M., Taurchini M., Ascani S, & Graziano P. (2020). Diagnosis of Hodgkin Lymphoma from Cell Block: A Reliable and Helpful Tool in “Selected” Diagnostic Practice. Diagnostics, 10(10), 748.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Melanoma Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Briefly, formalin fixed, paraffin embedded sections were deparaffinised, rehydrated, blocked for endogenous peroxidases and underwent antigen retrieval according to antibody specifications. Tissues were incubated overnight with the following primary antibodies. Anti-human melan-a clone A103 (Dako, M7196), S100 (Dako, Z0311), β-catenin (BD Transduction Laboratories, 610154), CyclinD1 clone EP12 (Dako, M3642), c-myc clone 9E10 (Santa Cruz Biotechnology, sc-40), p44/42 MAPK (Erk1/2) (Cell Signaling, 4695) and phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Cell Signaling, 9101), mouse IgG (Vector, I-2000), and rabbit IgG (Vector, I-1000). Secondary antibodies used for DAB based IHC were either EnVision+ System- HRP Labelled Polymer Anti-mouse (Dako, K4001) or EnVision+ System- HRP Labelled Polymer Anti-rabbit (Dako, K4003) based on primary antibody host species. Peroxidase activity was revealed using DAB (Dako, K3468). Samples were then counterstained with haematoxylin, dehydrated, and coverslipped.

Pawlikowski J.S., Brock C., Chen S.C., Al-Olabi L., Nixon C., McGregor F., Paine S., Chanudet E., Lambie W., Holmes W.M., Mullin J.M., Richmond A., Wu H., Blyth K., King A., Kinsler V.A, & Adams P.D. (2015). Acute Inhibition of MEK Suppresses Congenital Melanocytic Nevus Syndrome in a Murine Model Driven by Activated NRAS and Wnt Signaling. The Journal of Investigative Dermatology, 135(8), 2093-2101.

+ Open protocol

+ Expand

Immunohistochemistry of Formalin-Fixed Paraffin-Embedded Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols

Briefly, formalin fixed, paraffin embedded sections were deparaffinised, rehydrated, blocked for endogenous peroxidases, and underwent antigen retrieval according to antibody specifications. Tissues were incubated overnight with the following primary antibodies: Anti-human melan-a clone A103 (Dako, M7196), S100 (Dako, Z0311), β-catenin (610154, BD Transduction Laboratories, San Jose, CA), CyclinD1 clone EP12 (Dako, M3642), c-myc clone 9E10 (Santa Cruz Biotechnology, sc-40), p44/42 MAPK (Erk1/2; Cell Signaling, 4695) and phospho-p44/42 MAPK (Erk1/2, Thr202/Tyr204, Cell Signaling, 9101), mouse IgG (I-2000, Vector, Burlingame, CA), and rabbit IgG (Vector, I-1000). Secondary antibodies used for DAB-based IHC were either EnVision+ System- horseradish peroxidase Labeled Polymer Anti-mouse (Dako, K4001) or EnVision+ System- horseradish peroxidase Labeled Polymer Anti-rabbit (Dako, K4003) based on primary antibody host species. Peroxidase activity was revealed using DAB (Dako, K3468). Samples were then counterstained with haematoxylin, dehydrated, and coverslipped.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!