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2 protocols using anti inos and senescence detection kit

1

Cell Metabolism and Senescence Analysis

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The materials used in this study are the following: Dulbecco’s modified Eagle medium (DMEM)/F12, Penicillin-Streptomycin (P/S), 0.25% trypsin-EDTA, and 10% Fetal Bovine Serum (FBS) from Gibco BRL (Grand Island, NY, USA); Tween® 20 and ECLTM Western blotting detection reagent from Amersham Life Science (Arlington Heights, IL, USA); anti-β Actin from Sigma (St. Louis, MO, USA); anti-iNOS and senescence detection kit from Abcam (Cambridge, UK); Agilent Seahorse XF Cell Mito Test Kit from Agilent Technologies (CA, USA); Alexa Fluor® 594 conjugated Escherichia coli (K-12 strain) BioParticles® from Thermo Fisher Scientific (MA, USA); Tetramethylrhodamine Methyl Ester (TMRM) from Thermo Fisher Scientific.
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2

Mitochondrial Function and Senescence Assays

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The materials used in this study are as follows: Dulbecco’s modified Eagle medium (DMEM)/F12, Penicillin-Streptomycin (P/S), 0.25% trypsin-EDTA, and 10% Fetal Bovine Serum (FBS) were from Gibco BRL (Grand Island, NY, USA); Tween® 20 and ECLTM Western blotting detection reagent were from Amersham Life Science (Arlington Heights, IL, USA); anti-β Actin was obtained from Sigma (St. Louis, MO, USA); anti-iNOS and senescence detection kit were the product of Abcam (Cambridge, UK); minoxidil was obtained from Sigma; Agilent Seahorse XF Cell Mito Test Kit was from Agilent Technologies (CA, USA); Alexa Fluor® 594 conjugated Escherichia coli (K-12 strain) BioParticles® and Tetramethylrhodamine Methyl Ester (TMRM) were purchased from Thermo Fisher Scientific (MA, USA); MitoSOX™ was from Invitrogen (MA, USA).
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