Dulbecco’s modified Eagle’s medium (DMEM) and penicillin-streptomycin solution (100×) were from Gibco (Life Technologies, CA, USA). Fetal calf serum (FBS) was from Biological Industries (Kibbutz Beit-Haemek, Israel). Florfenicol was from Aladdin (Shanghai, China). DMSO, D-galactose and N-acetyl-L-cysteine (NAC) were from Sigma (St. Louis, MO, USA). AMPK inhibitor (compound C) and Bafilomycin A1 (Baf-A1) were from Selleck Chemicals (Houston, TX, USA).
Fetal calf serum fbs
Manufactured by Sartorius
Sourced in Israel
Fetal calf serum (FCS) is a common supplement used in cell culture media. It is derived from the blood of fetal calves and provides a complex mixture of proteins, growth factors, and other components that support the growth and maintenance of various cell types in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Penicillin streptomycin solution 100, by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
N acetyl l cysteine nac, by Merck Group (1 mentions)
D galactose, by Merck Group (1 mentions)
Ampk inhibitor compound c, by Selleck Chemicals (1 mentions)
Bafilomycin a1 baf a1, by Selleck Chemicals (1 mentions)
Penicillin streptomycin, by Sartorius (1 mentions)
Apoptosis detection kit, by Beyotime (1 mentions)
Smad4, by Proteintech (1 mentions)
Trypsin edta, by Sartorius (1 mentions)
β actin, by Proteintech (1 mentions)
Tnf α, by Cell Signaling Technology (1 mentions)
Smad2, by Proteintech (1 mentions)
Gli 1 antibody, by Abcam (1 mentions)
α sma, by Proteintech (1 mentions)
α minimum essential medium α mem, by Thermo Fisher Scientific (1 mentions)
β actin 66009 1 ig, by Proteintech (1 mentions)
4 protocols using fetal calf serum fbs
1
Pharmacological modulation of AMPK and autophagy
2
Transgenic Mouse Model for Liver Fibrosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Green fluorescence transgenic male C57Bl/6 mice (n = 20) and normal male C57Bl/6 mice (n = 36) 2343 bought from Gem Pharmatech Biotechnology Co., LTD (Jiangsu, China), with certificate No.320727210100567527. The DMEM high-sugar medium, fetal calf serum (FBS), penicillin-streptomycin, phosphate buffer (PBS), trypsin–EDTA were bought from Biological Industries (Israel). The Gli1 antibody was bought from Abcam (Cambridge, England). α-SMA, SMAD2, SMAD4, β-actin and goat anti-rabbit IgG II were bought from Proteintech Group Inc. (Rosemont, USA). The antibodies of CD44, CD177 and CD34 were bought from Cell Signaling Technology, Inc. (Boston, USA); TNF-a. IL-1, IL-1B, BUN and Scr ELISA kit were bought from Bluef (Shanghai, China) Biotechnology Development Co., LTD. (Shanghai, China). The apoptosis detection kit was bought from Beyotime Biotechnology (Shanghai, China).
3
Stem Cell Culture Reagents
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Minimum essential medium α (αMEM) and penicillin–streptomycin solution (100×) were from Gibco (Life Technologies, CA). Bovine calf serum (CS) and fetal calf serum (FBS) were from Biological Industries (Kibbutz BeitHaemek, Israel). FLO was from Aladdin (Shanghai, China). DMSO was from Sigma (St. Louis, MO, United States). Sox2 (66411-1-Ig), Oct4 (11263-1-AP), β-catenin (66379-1-Ig), and β-actin (66009-1-Ig) were from Proteintech (Wuhan, China).
4
HT-29 Cell Antioxidant Assays with Streptococcus
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The HT-29 human colonic epithelial cell line was used in this study. The cells were grown in RPMI 1640 medium (Hyclone, Logan, UT) containing 10% (vol/vol) fetal calf serum (FBS; Biological Industries, Kibbutz Beit Haemek, Israel), 1% (vol/vol) penicillin-streptomycin solution (100 U/mL of penicillin and 100 µg/ mL of streptomycin, Hyclone) at 37°C in a humidified atmosphere in the presence of 5% CO 2 . After reaching 80% confluence, the cells were detached using 0.25% trypsin-EDTA (Hyclone) and subsequently seeded in 6-well culture plates at a density of 8 × 10 5 cells/well for the antioxidant assays. After 24 h growth, cells were washed twice with PBS (Hyclone) and then co-incubated with 1 × 10 8 cfu/mL of S. thermophilus strains (optical density at 600 nm of 0.6-0.8) in FBS-free medium for 2 h under the conditions described above for the following experiments. Cells in fresh medium without FBS were regarded as control.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!