PLGA 75:25 (Resomer® RG 752 H, Mw = 4–15 kDa) (analytical grade), SC, PVA (Mw = 31–50 kDa, 98–99% hydrolyzed), PTM isethionate (PTM-I), dimethyl sulfoxide (DMSO), phosphoric acid, sodium hydroxide and sodium 1-heptanesulfonate were purchased from Merck (Milan, Italy). All the solvents used were of analytical grade or HPLC grade and were purchased from Carlo Erba Reagenti (Milan, Italy). Ultrapure water used for the buffers was obtained from a Milli-Q® Plus Purification System (Merck Millipore, Vimodrone Milan, Italy). Solvent evaporation was carried out using a rotating evaporator (Heidolph Laborota 400, Heidolph Instruments, Schwabach, Germany) equipped with a vacuum pump (Diaphragm Vacuum Pump DC-4). Lyophilization was performed with a LyoQuest-85® freeze drier (Azbil Telstar Technologies, Barcelona, Spain). C2C12 myoblasts, an immortalized murine cell line, were purchased from ECACC 91031101. Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), glutamine, amphotericin B and penicillin-streptomycin were purchased from Gibco, Thermo Fisher Scientific (Waltham, MA, USA). Thiazolyl Blue Tetrazolium Bromide (MTT solution), Phalloidin-Atto 488, Phalloidin-Atto 594 and Alexafluor 488 were obtained from Merck.
Milli q plus purification system
Manufactured by Merck Group
Sourced in United States, France, Germany, Italy
The Milli-Q Plus purification system is a water purification device designed to produce high-quality, ultrapure water for use in a variety of laboratory applications. The system utilizes a multi-stage filtration process to remove impurities, microorganisms, and other contaminants from the input water supply, resulting in water that meets the stringent purity requirements of critical laboratory procedures.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Merck Group (6 mentions)
Hplc grade acetonitrile, by Merck Group (6 mentions)
Formic acid, by Merck Group (6 mentions)
Sodium hydroxide (naoh), by Merck Group (3 mentions)
Mucin from porcine stomach type 2, by Merck Group (3 mentions)
Methanol, by Avantor (3 mentions)
Orthophosphoric acid, by Merck Group (3 mentions)
Acetonitrile, by Merck Group (3 mentions)
Estradiol, by Merck Group (2 mentions)
Cyclophosphamide, by Merck Group (2 mentions)
Fluconazole, by Merck Group (2 mentions)
Polyoxypropylene 5 polyoxyethylene 20 cetyl alcohol ppg 5 ceteth 20, by Croda (2 mentions)
Amphotericin b, by Merck Group (2 mentions)
Carbopol 974p, by Lubrizol (2 mentions)
Polycarbophil, by Lubrizol (2 mentions)
Formic acid hcooh, by Merck Group (2 mentions)
Trifluoroacetic acid, by Merck Group (2 mentions)
Dimethyl sulfoxide d6, by Merck Group (2 mentions)
Acetonitrile, by Avantor (2 mentions)
Deuterated chloroform, by Merck Group (2 mentions)
61 protocols using milli q plus purification system
1
Biodegradable PLGA Nanoparticle Formulation
2
Surfactant-based Methotrexate Delivery System
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The surfactant polyether functional siloxane (PFS) is available commercially as DC®5329 (Dow Corning, Midland, MI, USA). Silicone glycol copolymer fluid (SFGC) is available commercially as DC®193 Fluid (Dow Corning), and methotrexate was acquired from Genix (São Paulo, Brazil). Carbomer homopolymer type A (C971) is available commercially as Carbopol® 971P NF Polymer (Lubrizol, Cleveland, OH, USA). High-purity water was prepared with a Milli-Q plus purification system (Merck Millipore, Darmstadt, Germany), 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and phosphate buffered saline (PBS) were purchased from Sigma (St. Louis, MO, USA). Dimethyl sulfoxide (DMSO) was purchased from Viafarma (São Paulo, Brazil).
3
Topical Formulation Development and Evaluation
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Polyoxypropylene (5) polyoxyethylene (20) cetyl alcohol (PPG-5-CETETH-20) was purchased from Croda (Campinas, Sao Paulo, Brazil). Oleic acid (OA) was purchased from Synth (Diadema, Sao Paulo, Brazil). Polycarbophil® and Carbopol® 974P were purchased from Lubrizol® AdvancedMaterials (Cleveland, OH, USA). High-purity water was prepared with a Millipore Milli-Q Plus purification system (EMD Millipore, Billerica, MA, USA); its resistivity was 18.2 MΩ-cm. Mucin from porcine stomach type II, amphotericin B, fluconazole, estradiol, cyclophosphamide, 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) sodium salt, triphenyltetrazolium chloride, methanol and acid 3-(N-morfolino) propanosulfônic were purchased from Sigma-Aldrich Co. (St Louis, MO, USA).
4
Quantitative Analysis of Antimicrobials
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United States Pharmacopeia reference standards of VAN, MER, PIP, TAZ, and cefotaxime (CTX) and other chemicals were purchased from Sigma-Aldrich (Milan, Italy). Organic solvents (HPLC grade) were obtained from Carlo Erba (Milan, Italy).
Blank plasma was obtained from patients undergoing plasmapheresis at the Molinette Hospital of Turin, Italy.
Ultrapure water used for the buffers was obtained from a Milli-Q Plus Purification System (Merck Millipore, Vimodrone Milan, Italy). All the buffers were passed through a 0.22-μm membrane filter before use.
Blank plasma was obtained from patients undergoing plasmapheresis at the Molinette Hospital of Turin, Italy.
Ultrapure water used for the buffers was obtained from a Milli-Q Plus Purification System (Merck Millipore, Vimodrone Milan, Italy). All the buffers were passed through a 0.22-μm membrane filter before use.
5
HPLC Analysis of Mycotoxin Standards
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The following reagents were used for the analysis: standard sterigmatocystin, high-performance liquid chromatography (HPLC) grade acetonitrile (Sigma-Aldrich, USA), standard roquefortine C (LCG Standards, Germany), sodium acetate, acetic acid (POCH S.A., Poland), HPLC grade methanol (JT Baker, Netherlands) and water purified by using Milli-Q Plus Purification System (Merck Millipore, Germany). Qualitative and quantitative analyses were conducted using a Waters Alliance HPLC system equipped with Waters 2695 separation module, Waters 2996 PAD detector, Jet Stream column thermostat and Empower 2 software or Waters Breeze HPLC system equipped with Waters 1525 binary pump, Waters 717 plus autosampler, Waters 2487 dual absorbance detector, Jet Stream column thermostat and Breeze software. Chromatographic separations were carried out using C-18 Waters Symmetry (150×2.1 mm, 5 μm) analytical column eluted (gradient elution) with methanol:acetate buffer mobile phase.
6
Dacomitinib Metabolism Analysis in Rat Liver
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HPLC grade solvents were utilized throughout the entire study. Dacomitinib reference standard was acquired from Med Chem. Express (Princeton, NJ, USA). Acetonitrile (ACN), ammonium formate (NH4COOH), potassium cyanide (KCN), methoxyl amine (MeONH2) and formic acid (HCOOH) were procured from Sigma-Aldrich (USA). Water (ultrapure) was taken from in-house Milli-Q plus purification system (Millipore, Midford, MA USA). Rat liver microsomes (RLMs) were acquired in-house using Sprague Dawley rats.33–36 (link) The used rats were attained from the experimental animal care center at college of Pharmacy, King Saud University (KSA). The University's Ethics Review Committee approved the animal experimental design.
7
Comprehensive Phytochemical Analysis of SMT
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SMT was purchased
from Hunan Hansen Pharmaceutical Co., Ltd. (Yiyang, Hunan Province,
China), which was prepared according to the preparation process of
Chinese Pharmacopoeia (2015 edition). HPLC grade acetonitrile, methanol,
and formic acid used in this study were purchased from Fisher Scientific.
High pure water was prepared by using a Millipore Milli Q plus purification
system. All other reagents were analytical grade and commercially
available. Reference standards of valine, guvacoline, arecoline, phenylalanine,
7-hydroxycoumarin, narirutin, naringin, neohesperetin, hesperetin,
linderane, costunolide, isoalantolactone, helenine, and nobiletin
were purchased from the National Institute for the Control of Pharmaceutical
and Biological Products (Beijing, China). All standards were of at
least 98% purity and were suitable for UHPLC-LTQ-Orbitrap analysis.
from Hunan Hansen Pharmaceutical Co., Ltd. (Yiyang, Hunan Province,
China), which was prepared according to the preparation process of
Chinese Pharmacopoeia (2015 edition). HPLC grade acetonitrile, methanol,
and formic acid used in this study were purchased from Fisher Scientific.
High pure water was prepared by using a Millipore Milli Q plus purification
system. All other reagents were analytical grade and commercially
available. Reference standards of valine, guvacoline, arecoline, phenylalanine,
7-hydroxycoumarin, narirutin, naringin, neohesperetin, hesperetin,
linderane, costunolide, isoalantolactone, helenine, and nobiletin
were purchased from the National Institute for the Control of Pharmaceutical
and Biological Products (Beijing, China). All standards were of at
least 98% purity and were suitable for UHPLC-LTQ-Orbitrap analysis.
8
Arsenic Speciation in Traditional Medicine
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NHJDT (Batch No.151221090, 0.27 g/tablet) were purchased from Beijing Tong Ren Tang Technologies Co., Ltd (Beijing, China). Arsenic trioxide and the arsenate reference standard were provided by National Institutes for Food and Drug Control (Beijing, China). Dimethylarsenic acid (DMA) was obtained from Huamai Biotechnology Co., Ltd (Beijing, China). Monosodium methanearsonicacid (MMA) was purchased from Laboratories of Dr. Ehrenstorfer GmbH (Augsburg, Germany). Thiourea, ascorbic acid, and potassium borohydride of analytical grade and hydrochloric acid, nitric acid, and sulfuric acid of guaranteed grade were all supplied by Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Potassium hydroxide, potassium dihydrogen phosphate, and potassium sulfate were provided from Nanjing Chemical Reagent Co., Ltd. (Nanjing, China). HPLC grade methanol was from Tedia Company, Inc (Fairfield, United States). All the other chemicals were of analytical reagent grades. Deionized water was purified on a Millipore Milli Q-Plus purification system (Millipore, MA, United States).
9
Synthesis and Characterization of Isoproterenol Hydrochloride
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The samples of isoproterenol hydrochloride obtained from different final step batches, reference standard of isoproterenol hydrochloride (purity 99.9%) and the starting material, 1-(3,4-bis(benzyloxy)phenyl)-2-(isopropylamino)ethanone hydrochloride (2) used in the synthesis, were provided by Chemical Research Division, Micro Labs Ltd. (Bangalore, India). The catalyst, Pd-C (50% wet), was acquired from Monarch Catalyst Private Limited (Mumbai, India). HPLC grade acetonitrile and methanol were purchased from Spectrochem Pvt Ltd. (Bangalore, India), and formic acid and trifluoroacetic acid were purchased from Sigma-Aldrich (Bangalore, India). The commercial grade ethyl acetate (EtOAc) and isopropyl alcohol (IPA) were procured from Taiwan Fieldrich Corporation (Taipei, China). Purified water by Millipore Milli-Q Plus Purification System (Bradford, PA, USA) was used during the course of experimental studies. CD3OD was purchased from Cambridge Isotope Laboratories, Inc. (Andover, MA, USA). Potassium bromide used in Fourier transform infrared spectroscopy (FT-IR) was purchased from Merck KGaA (Darmstadt, Germany). TLC plates (60 F254) procured from Merck (Delhi, India) was used to check the progress of reaction.
10
Formulation and Evaluation of Topical Antifungal Gel
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Polyoxypropylene (5) polyoxyethylene (20) cetyl alcohol (PPG-5-CETETH-20) was purchased from Croda (Campinas, Sao Paulo, Brazil). Oleic acid was purchased from Synth (Diadema, Sao Paulo, Brazil). Polycarbophil® and Carbopol® 974P was purchased from Lubrizol® (Wickliffe, OH, USA) Advanceds materials (Cleveland, OH, USA). Sterile fetal bovine serum was purchased from Laborclin® (Pinhais, Paraná, Brazi) laboratory products, Pinhais, Paraná-Brazil. The high-purity water was prepared with a Millipore Milli-Q Plus purification system, and its resistivity was 18.2 MΩ-cm. Sabouraud Dextrose Ágar, Both and supplemented with chloramphenicol were purchased from Difco®-Becton (Franklin Lakes, NJ, USA) Dickinson and Company Sparks (Le Pont de Claix, France). Methanol was purchased from Merck® (Darmstadt, Germany). Triethanolamine, Mucin from porcine stomach type II, amphotericin B, fluconazole, estradiol, cyclophosphamide, XTT sodium salt, triphenyltetrazolium chloride (TTC), gentamicin were purchased from Sigma Aldrich® (Steinheim, North Rhine-Westphalia, Germany).
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