Horseradish peroxidase hrp conjugated goat anti rabbit and goat anti mouse igg antibodies

Mouse anti-dsRNA mAb (clone J2) was obtained from SCICONS. Polyclonal rabbit anti-SARS-CoV-2 nucleocapsid antibody was from Novus. Mouse anti-HCV E1 mAb A4 (Dubuisson et al., 1994 (link)) was produced in vitro. Mouse anti-GFP mAb was from Roche. Rabbit anti-calnexin antibody was from Abcam. Rabbit anti-ACE2 antibody was from Cell Signaling Technology. Mouse anti-transferrin receptor mAb was from SantaCruz. Cyanine 3-conjugated goat anti-mouse IgG and horseradish peroxidase (HRP)-conjugated goat anti-rabbit and goat anti-mouse IgG antibodies were from Jackson ImmunoResearch.

Fetal bovine serum (FBS) was purchased from Hyclone (Logan, UT, USA). The following antibodies were purchased: Rac1 antibody (BD Biosciences, Franklin Lakes, NJ, USA); c-Jun N-terminal kinase (JNK), p-JNK, p-p38, p38, p-ERK, ERK, p-PKC, PKC, p-c-Src, c-Src, p-NF-κBp65, NF-κBp65, p-c-Jun, c-Jun, Bax, p-Bcl-2, Bcl-2, caveolin-1, cleaved caspase-3, caspase-9, and β-actin antibodies (Santa Cruz Biotechnology, Paso Robles, CA, USA); Bax (6A7) monoclonal antibody (Thermo Fisher Scientific, Rockford, IL, USA); LC-3, NCF-1 and Beclin-1 antibodies (Novus Biologicals, Littleton, CO, USA). VvhA-specific antibody was acquired from Professor Sang Ho Choi (Seoul National University, Seoul, Korea). Horseradish peroxidase (HRP)-conjugated goat anti-rabbit and goat anti-mouse IgG antibodies (Jackson Immunoresearch, West Grove, PA, USA). SP600125 was purchased from Sigma-Aldrich (St. Louis, MO, USA). All other reagents were of the highest purity commercially available and were used as received.

Fetal bovine serum (FBS) was purchased from BioWhittaker (Walkersville, MO, USA). The following antibodies were purchased: p-PKC and PKC antibodies (Cell Signaling Technology, Danvers, MA, USA); NOX2 antibody (BD Biosciences, Franklin Lakes, NJ, USA); NCF1 antibody (LifeSpan Biosciences, Seattle, WA, USA); ITLN and Na⁺/K⁺ ATPase antibodies (abcam, Cambridge, MA, USA); p-ERK1/2, ERK, p-JNK, JNK, p-p38 MAPK, p38 MAPK, p-c-Src, c-Src, p-FAK, FAK, pan-cadherin, PKCα, PKCδ, and PKCζ antibodies (Santa Cruz Biotechnology, Paso Robles, CA, USA); Horseradish peroxidase (HRP)-conjugated goat anti-rabbit and goat anti-mouse IgG antibodies (Jackson Immunoresearch, West Grove, PA, USA); 2′, 7′-dichlorofluorescein diacetate (CM-H₂DCFDA) was obtained from Invitrogen (Carlsbad, CA, USA). A23187, methyl-β-cyclodextrin (MβCD), N-acetyl-l-cysteine (NAC) and 5-azacytidine were purchased from Sigma Chemical Company (St. Louis, MO, USA). The concentrations of all of pharmacological inhibitors listed did not show any significant cytotoxic effects by themselves as confirmed by FACS analysis in each experiment. All other reagents were of the highest purity commercially available and were used as received.