Log In Sign up
The largest database of trusted experimental protocols

24 well modified boyden chambers transwell chamber

Manufactured by BD
Visit Supplier
Sourced in United States

The 24-well modified Boyden chambers (transwell-chamber) is a lab equipment used for in vitro cell migration and invasion assays. The chambers consist of an upper compartment separated from a lower compartment by a porous membrane, allowing cells to migrate from the upper to the lower chamber.

Automatically generated - may contain errors

Lab products found in correlation

Matrigel, by BD (3 mentions) Diff quik stain, by Sysmex (3 mentions)

3 protocols using 24 well modified boyden chambers transwell chamber

1

Transwell Assays for Cell Migration and Invasion

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Transwell migration and invasion assays were carried out in 24-well modified Boyden chambers (transwell-chamber, BD Transduction, Franklin Lakes, NJ, USA). For invasion assays, the upper surfaces of the 6.4-mm diameter filters with 8-μm pores that were used for migration assays were precoated with Matrigel (BD Transduction). siRNA transfectants (2 × 104 cells per well) were transferred into the upper chamber. Following 48 h of incubation, migrated or invasive cells on the lower surface of the filters were fixed and stained with the Diff-Quik stain (Sysmex, Kobe, Japan), and stained cell nuclei were counted directly in triplicate. We assessed the ability of the cells to move through extracellular matrices by calculating the number of cells, which is the ratio of the percentage invasion through the Matrigel-coated filters relative to migration through the uncoated filters of test cells over that in the control cells as described elsewhere (Kashimoto et al, 2012 (link); Nishimura et al, 2013 (link)).
Komatsu S., Ichikawa D., Hirajima S., Nagata H., Nishimura Y., Kawaguchi T., Miyamae M., Okajima W., Ohashi T., Konishi H., Shiozaki A., Fujiwara H., Okamoto K., Tsuda H., Imoto I., Inazawa J, & Otsuji E. (2014). Overexpression of SMYD2 contributes to malignant outcome in gastric cancer. British Journal of Cancer, 112(2), 357-364.
+ Open protocol
+ Expand
2

Transwell Migration and Invasion Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Transwell migration and invasion assays were carried out in 24-well modified Boyden chambers (transwell-chamber, BD Transduction, Franklin Lakes, NJ). The upper surface of the 6.4 mm diameter filters with 8-μm pores was pre-coated with (invasion assay) or without (migration assay) Matrigel (BD Transduction). The siRNA transfectants (2 × 104 cells per well) were transferred into the upper chamber. Following 48 h of incubation, migrated or invasive cells on the lower surface of filters were fixed and stained with the Diff-Quik stain (Sysmex, Kobe, Japan), and stained cell nuclei were counted directly in triplicate. We assessed the invasive potential by calculating the ratio of the percentage invasion through the Matrigel-coated filters relative to the migration through the uncoated filters of test cells over that in the control counterparts, as described elsewhere [37 (link)–40 (link)].
Aratani K., Komatsu S., Ichikawa D., Ohashi T., Miyamae M., Okajima W., Imamura T., Kiuchi J., Nishibeppu K., Kosuga T., Konishi H., Shiozaki A., Fujiwara H., Okamoto K., Tsuda H, & Otsuji E. (2017). Overexpression of CTEN relates to tumor malignant potential and poor outcomes of adenocarcinoma of the esophagogastric junction. Oncotarget, 8(48), 84112-84122.
+ Open protocol
+ Expand
3

Transwell Migration and Invasion Assay Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Transwell migration and invasion assays were carried out in 24-well modified Boyden chambers (transwell-chamber, BD Transduction, Franklin Lakes, NJ). The upper surface of the 6.4 mm diameter filters with 8-μm pores was pre-coated with (invasion assay) or without (migration assay) Matrigel (BD Transduction). The siRNA transfectants (2 × 104 cells per well) were transferred into the upper chamber. Following 48 h of incubation, migrated or invasive cells on the lower surface of filters were fixed and stained with the Diff-Quik stain (Sysmex, Kobe, Japan), and stained cell nuclei were counted directly in triplicate. We assessed the invasive potential by calculating the ratio of the percentage invasion through the Matrigel-coated filters relative to migration through the uncoated filters of test cells over that in the control counterparts [33 (link), 34 (link)].
Kobayashi H., Komatsu S., Ichikawa D., Kawaguchi T., Hirajima S., Miyamae M., Okajima W., Ohashi T., Kosuga T., Konishi H., Shiozaki A., Fujiwara H., Okamoto K., Tsuda H, & Otsuji E. (2015). Overexpression of denticleless E3 ubiquitin protein ligase homolog (DTL) is related to poor outcome in gastric carcinoma. Oncotarget, 6(34), 36615-36624.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!