Bicinchoninic acid bca protein assay kit

Cell Counting Kit-8 (CCK-8), Bicinchoninic acid (BCA) Protein Assay Kits, Annexin V-FITC Apoptosis Detection Kits, and Cell Cycle Analysis Kits were all purchased from the Beyotime Institute of Biotechnology (Jiangsu, China). Protein Extraction Kits were obtained from KEYGEN Biotech. Co., Ltd. (Nanjing, China). Diaminobenzidine (DAB) substrate kits were purchased from Zhongshan Golden Bridge Biotechnology (Beijing, China). Terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL) kits were provided by Roche Diagnostics (Germany). 4′,6′-Diamidino-2-phenylindole (DAPI), tris (hydroxymethyl) aminomethane (Tris), and sodium dodecyl sulphate (SDS) were purchased from Sigma (St. Louis, MO, U.S.A.). Anti-LOX1, anti-caspase-9, anti-p65, anti-p-IKKβ, anti-IKKβ, anti-p-IkBα, anti-IkBα, anti-GAPDH, anti-β-tubulin, and anti-Lamin B primary antibodies, and horseradish peroxidase-conjugated antibody were obtained from Abcam (Cambridge, U.K.). Plasmids harboring the wild-type caspase-9 response element (WT-luciferase-caspase-9) and the corresponding mutant (MUT-luciferase-caspase-9) were purchased from Vipotion Biotechnology (Guangzhou, China). Pierce Agarose Chip Kits were obtained from Thermo Fisher Scientific (Waltham, MA, U.S.A.).

Tanshinone IIA (purity > 99%) was obtained from Chengdu de Stewart Biological Technology Co., Ltd. (Sichuan, China). Streptozotocin (STZ) was purchased from Sigma (St. Louis, MO, USA). The following primary antibodies (Abcam, MA, UK) were used in the Western blotting analyses: TNF-α (ab6671), IL-1β (ab2105), IL-6 (ab9324), and IL-10 (ab33471); antibody against β-actin (sc-47778) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Enhanced chemiluminescence (ECL) detection reagents were obtained from HuaXingbio Centre of Biotechnology (Beijing, China); bicinchoninic acid (BCA) protein assay kits and RIPA lysis buffer were obtained from Beyotime Institute of Biotechnology (Haimen, China).

T-2 toxin was obtained from Cayman Chemical (Ann Arbor, MI, USA). MTT, AVPI, LDH, acridine orange (AO), Hoechst 33258 staining, and Bicinchoninic acid (BCA) protein assay kits were purchased from Beyotime (Nantong, China). AST, ALT, CAT, GSH-PX and MDA assay kits were purchased from Nanjing KeyGen Biotech. Co. Ltd. (Nanjing, China). Antibodies of mouse monoclonal anti-β-actin, rabbit monoclonal anti-Bcl-2, rabbit monoclonal anti-Bax, rabbit monoclonal anti-PARP-1, rabbit monoclonal anti-caspase-3, rabbit monoclonal anti-P62, rabbit monoclonal anti-Beclin 1, rabbit monoclonal anti-LC3, goat anti-rabbit IgG-HRP, and goat anti-mouse IgG-HRP were purchased from Proteintech (Rosemont, IL, USA). Rapamycin (RAPA) and chloroquine diphosphate salt (CQ) were obtained from Solarbio (Beijing, China). pGMLV-CMV-RFP-GFP-hLC3 lentivirus was purchased from Genomeditech (Shanghai, China).

Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin/streptomycin, and phosphate-buffered saline (PBS) were purchased from Gibco (Grand Island, NY, USA). Antibodies against β-Actin, NCOA4, FTH1, LC3, and GPX4, as well as HRP Goat Anti-Rabbit Ig G (H + L), were obtained from ABclonal (Wuhan, China). LPS, MT, 4-P-PDOT, luzindole were purchased from Sigma-Aldrich (St. Louis, MO, USA). Erastin and rapamycin were purchased from Selleck (Houston, Texas, USA). Bicinchoninic acid (BCA) protein assay kit was purchased from Beyotime Biotechnology (Shanghai, China).

The H9c2 cell line was obtained from ATCC (Manassas, VA,USA), sodium hydrogen sulfide (NaHS) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Dorsomorphin dihydrochloride (HY-13418) was obtained from MCE. Glucose, streptozotocin (STZ), dl-propargylglycine (PAG) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM 5.5 mM D-Glucose), penicillin, streptomycin and fetal bovine serum (FBS) were from Hyclone (Grand Island, NY), antibodies against SIRT6, AMPK, p-AMPK, LC3A/B, BECLIN1, ATG5, ATG16L1, P53, P21, P16 were from Boster Biological Technology (Ltd. Wuhan, China. Bicin choninic Acid (BCA) Protein Assay kit, Enhanced Chemiluminescence Reagent kit, SDS-PAGE Gel Preparation kit and phenyl methyl sulfonyl fluoride (PMSF) were purchased from Beyotime Institute of Biotechnology(Shanghai, China), SPiDER-βGal was purchased from dojindo (Japan).

Cell Counting Kit-8 (CCK-8; CK04–50000 T; Dojindo, Kumamoto, Japan), Annexin V-FITC/propidium iodide (PI) kit (AD10-50 T; Dojindo, Japan), dimethyl sulfoxide (DMSO; 1129E033; Solarbio, Beijing, China), foetal bovine serum (FBS; 10,099; Gibco, Waltham, MA, USA), trypsin-EDTA solution (25,200,072; Gibco), Dulbecco's modified Eagle medium (DMEM; 11054001, Gibco), RPMI 1640 medium (A1049101; Gibco), Leibovitz's L15 medium (11415064; Gibco), rapamycin (S1039; Selleckchem, Houston, TX, USA), 2.5% glutaraldehyde fixation (DF0156, Leagene Biological, Beijing, China), and bicinchoninic acid (BCA) protein assay kit (Beyotime Institute of Biotechnology, Shanghai, China) were used. The β-actin (13E5), cyclin B1 (D5C10), cyclin A2 (E6D1J), phospho-cdc2 (Tyr15) (10A11), cyclin-dependent kinase 2 (CDK2; E8J9T), phospho-histone H2A.X (Ser139) (D7T2V), GAPDH (D16H11), Bax (2D2), cleaved PARP (Asp214) (D64E10), vimentin (D21H3), E-cadherin (4A2), p38 MAPK (D13E1), phospho-p38 MAPK (Thr180/Tyr182) (D3F9), SAPK/JNK antibody, phospho-SAPK/JNK (Thr183/Tyr185) (G9), Akt (pan) (C67E7), Phospho-Akt (Ser473) (D9E), p44/42 MAPK (Erk1/2) (137F5), and phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) were all purchased from Cell Signalling Technology (CST; Danvers, MA, USA).