Ovaries of each mouse got fixed with 4% formaldehyde, dehydrated in ethanol, embedded with paraffin, and sliced into 4 µm sections. Subsequently, the sections were stained with the adoption of commercial H&E kits (Solarbio, Beijing, China). Under an Olympus CX21 microscope (Olympus, Japan), the images of ovaries were visualized [20 (link)].
H e kit
Manufactured by Solarbio
Sourced in China
The H&E kit is a laboratory equipment used for the preparation and staining of tissue samples. It contains the necessary reagents and tools for the hematoxylin and eosin (H&E) staining technique, which is a commonly used method in histology and pathology for the visualization of cellular and tissue structures.
Automatically generated - may contain errors
Lab products found in correlation
Inverted microscope, by Olympus (5 mentions)
Goat serum, by Solarbio (5 mentions)
Mayer hematoxylin solution, by Solarbio (4 mentions)
Abc peroxidase and diaminobenzidine, by ZSGB-BIO (4 mentions)
Biotinylated secondary antibody, by ZSGB-BIO (3 mentions)
Cx21 microscope, by Olympus (1 mentions)
Rhodamine b isothiocyante rbitc, by Maclin Biochemical Technology (1 mentions)
Dextran sulfate sodium (dss), by MP Biomedicals (1 mentions)
Sodium alginate, by Merck Group (1 mentions)
Chitosan, by Merck Group (1 mentions)
Dp70 microscope, by Olympus (1 mentions)
Rm2255, by Leica (1 mentions)
Dm1000, by Leica (1 mentions)
Potassium ferrocyanide, by Merck Group (1 mentions)
Streptavidin peroxidase kit, by ZSGB-BIO (1 mentions)
Dab kit, by Solarbio (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
25 protocols using h e kit
1
Histological Analysis of Mouse Ovaries
2
Resveratrol-Loaded Chitosan-Alginate Nanoparticles
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PLGA (lactide: glycolide = 50:50, ester terminated, Mw = 38,000−54,000), Polyvinylalcohol (PVA, 86.5–89% hydrolyzed, viscosity 4.6–5.4 mPa·s) and resveratrol were purchased from Aladdin (Shanghai, China). Chitosan (molecular weight, 50,000–190,000; viscosity, 20–30 cP; and deacetylation 75%) and sodium alginate (low viscosity, 80,000–120,000; molecular weight, viscosity 2000 cP) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dextran sodium sulfate (DSS) was supplied by MP Biomedicals (Irvine, CA, USA). HE kits were purchased from Solarbio (Beijing, China). Male C57BL/6 mice (eight-week-old, 18–22 g) were provided by Pengyue Laboratory Animal Technology Co., Ltd. (Jinan, China), and preserved in an aseptic environment. Rhodamine B isothiocyante (RBITC) was purchased from Macklin (Shanghai, China). All other materials are analytical grade. Distilled water is used throughout the process.
3
Histological Analysis of Ileum Tissue
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HE staining were performed using HE kit (G1120; Solarbio, Beijing, China) according to the manufacturer’s instructions. Briefly, samples for histopathology were removed from the ileum region and placed into neutral buffered formalin for fixation, and 5 μm thick HE-stained sections were prepared following paraffin embedding and histological processing. Finally, images were taken using a DP70 microscope (Olympus, Tokyo, Japan).
4
Brain Tissue Histological Staining
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Brain slices were stained with an H&E kit (Solarbio, China) according to the manufacturers' instructions.
5
Histological Brain Tissue Processing
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After anesthetization, mice brains were gently removed and fixed in 4% paraformaldehyde overnight at 4°C. Brain tissues were then progressively dehydrated and embedded in paraffin. Coronal sections (8 μm) were cut using a microtome (Leica RM2255). Finally, these tissue sections were stained with an H&E kit (Solarbio, Beijing, China) according to the manufacturers' instructions.
6
Histological Analysis of Iron Deficiency
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A long-term iron-deficient diet causes the imbalance of liver function and iron loss. In this research, hepatic tissues fixed in 4% paraformaldehyde solution were dehydrated, embedded in paraffin, and cross-sectioned into 4-μm thick slices. The slices were then treated with HCl (5%) to release ferric ions, followed by treatment with 5% potassium ferrocyanide (Sigma, MO, USA) to produce insoluble ferric ferrocyanide and counterstained with the neutral red (18 (link), 19 (link)). In addition, the H&E staining of colon tissue was carried out according to the previous method (20 (link)). Briefly, colon tissues were cut into 5 μm-thick sections and stained with the H&E kit (Solarbio Science and Technology, Beijing, China). All the stained samples were observed under the microscope (DM1000, Leica Microsystems, Wetzlar, Germany).
7
Histological Analysis of Heart Tissues
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Heart tissues were fixed in 4% paraformaldehyde for 24 h at 4 °C. The tissues were then embedded in paraffin, cut into 5 μm sections in thickness, and placed on glass slides. The paraffin slides were dewaxed, rehydrated, and stained with H&E Kit (Solarbio, Beijing, China). For the IHC staining, the paraffin slides were dewaxed, rehydrated, and subjected to antigen retrieval in sodium citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 9.0). IHC was performed using a streptavidin-peroxidase kit (ZSGB-BIO, Beijing, China) according to the manufacturer’s instructions. After visualization with a DAB kit (Solarbio, Beijing, China), the slides were counterstained with hematoxylin and sealed with neutral resin.
8
Histological Analysis of Rat Penis
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After the ICP experiment, the rat penises were harvested. Three penises tissue in each group were fixed with 4% polyformaldehyde for 4 h, then embedded in optimal cutting temperature compound (OCT), cut into 5-μm thick sections, and mounted on glass slides. For H&E staining, the slices were stained with hematoxylin-eosin (H&E) kit (Solarbio Science & Technology Co., Ltd., Beijing, China) according to the manufacturer's protocol. The result was shown in Figure S2 .
9
Immunohistochemical Analysis of Autophagy
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Samples from Area II were fixed, paraffin-embedded, and sliced into 5 µm thick slides. Then, the hematoxylin and eosin (H&E) Kit (Solarbio Science & Technology) was used to stain the slides.
For IHC, slides were first deparaffinized and rehydrated. After blocking thec endogenous peroxidase, these slides were incubated with primary antibodies against LC3 (1:100), Parkin (1:200), TFEB (1:100), SOD1 (1:200), HO-1 (1:200) overnight at 4 °C. After washing three times, these slides were incubated with a secondary antibody for 1 h at 37 °C. Finally, images were captured under an optical microscope and analyzed with the Image Pro-plus software.
For IHC, slides were first deparaffinized and rehydrated. After blocking thec endogenous peroxidase, these slides were incubated with primary antibodies against LC3 (1:100), Parkin (1:200), TFEB (1:100), SOD1 (1:200), HO-1 (1:200) overnight at 4 °C. After washing three times, these slides were incubated with a secondary antibody for 1 h at 37 °C. Finally, images were captured under an optical microscope and analyzed with the Image Pro-plus software.
10
Cell Culture and Assay Reagents
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DMEM culture medium, Fetal bovine serum, Penicillin, Streptomycin and Trypsin (All purchased from Gibco, USA); Fibronectin (Sigma, USA), Stem cell growth factor (Sigma, USA), Percoll (Sigma, USA), Oxazolone (Sigma, USA), Compound 48/80 (Sigma, USA). Rutin and hyperin (Yuanye, Shanghai). H&E kit (Solarbio, Beijing), Toluidine Blue (Aladdin, Shanghai).
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