Unicel dxc 800 synchron

The main independent variable was SUA measured in mg/dL. SUA levels were collected during subject enrolment in the NHANES using a colorimetric method in which uricase oxidises UA to allantoin and hydrogen peroxide (the Beckman Coulter UniCel DxC 800 Synchron chemistry analyzer between 2013 to 2014, the Beckman Coulter UniCel DxC 800 Synchron and the Beckman Coulter UniCel DxC 660i Synchron since 2015). Quality-control procedures' specifics have been disclosed elsewhere [29]. Values are reported in mg/dL and can be converted to μmol/L by multiplying by 59.48.

In NHANES 2011–2012, serum specimens were processed, stored, and shipped to the Collaborative Laboratory Services for analysis. Detailed specimen collection and processing instructions were available in the NHANES Laboratory/Medical Technologists Procedures Manual (LPM). Vials were stored under appropriate frozen (-30°C) conditions until they were shipped to the National Center for Environmental Health for testing. SCr was measured by the Jaffé rate method (kinetic alkaline picrate) using a Beckman Coulter UniCel® DxC800 Synchron at the Collaborative Laboratory Services at Ottumwa, Iowa, in 2011–2012.
For urine Cr analysis, spot or timed urine samples were stored at 2–8°C until analysis within 36 hours of receipt in the laboratory. Urine Cr was measured by an enzymatic (creatinase) method with Roche/Hitachi Modular P Chemistry Analyzer. For urine osmolality, spot or timed samples were analyzed directly at the mobile examination center within 4 hours of collection. Urine osmolality was measured by the freezing point depression method with Osmette II, Model 5005 Automatic Osmometer.

We collected participant information on age, sex and race-ethnicity (including Mexican American, non-Hispanic white, and non-Hispanic black). Smoking status was categorized as “non-smoker” for subjects who never smoked and “smoker” for subjects who were ex-smokers or current smokers by asking the question, “Do you now smoke cigarettes?” Alcohol drinking was determined through the participants’ self-reports. Co-morbidities, including asthma, congestive heart failure, coronary heart disease, angina, and stroke, were ascertained by self-reports. The total IgE level was measured using anti-IgE, which was covalently coupled to the ImmunoCap™ reaction vessel. CRP levels were measured by latex-enhanced nephelometry using the Behring Nephelometer System. Total bilirubin levels were evaluated by biochemical profiling. The levels of total cholesterol, triglycerides, and HDL-C were assessed using the Hitachi-704 analyzer. Creatine was assessed using the Beckman UniCel^® DxC800 Synchron. Fasting glucose was assessed using the Hexokinase-mediated reaction Roche/Hitachi Cobas C Chemistry Analyzer. Insulin was assessed with human insulin immunoassay by using the Molecular Devices, SpectraMax 250. Vitamin B12 are measured by using the Bio-Rad Laboratories.