Z0334
Z0334 is a lab equipment product. It is designed to facilitate a specific laboratory function. The core function of this product is to assist in the execution of experimental procedures within a laboratory setting.
Lab products found in correlation
13 protocols using z0334
Astrocyte Immunofluorescence Staining Protocol
Western Blot Analysis of Astrocyte Proteins
Histological Analysis of Ischemic Stroke in Mice
Immunolabeling of Neurogenic Markers in Brain
Immunofluorescence Staining of Mouse Brain
Free-floating brain slices were washed 5 min in PBS and blocked and permeabilized in PBS, BSA 1% and 0,3% Triton X-100 solution for 20 min. After two washes of 5 min with PBS (0.1 M), primary antibody (GFAP (Dako/Z0334) or Iba-1 (Abcam/ab48050), were incubated over-night at 4ºC at a dilution of 1:400. The following day, after two washes with PBS, the secondary antibody (Alexa Fluor 594, Abcam/ab150080) was incubated at room temperature for 1 h in the dark at a dilution of 1:400. Later, sections were co-incubated with 1 mg/mL Hoechst (Sigma) staining solution for 5 min in the dark at room temperature and washed twice for 5 min in PBS. Finally, the slices were mounted with Fluoromount G (EMS, USA).
Retinal Immunofluorescence Staining Protocol
Cryo-Tissue Analysis of Kainic Acid Injury
Immunocytochemistry Protocol for Oligodendrocyte Lineage
Cerebral Organoid Tissue Processing and Immunostaining
Immunocytochemistry of Neuronal and Glial Cultures
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