Cytofix cytoperm plus kit with golgiplug
The BD Cytofix/Cytoperm Plus Kit (with BD GolgiPlug) is a laboratory reagent used for the intracellular staining and flow cytometric analysis of proteins. The kit includes a fixation and permeabilization solution, as well as a protein transport inhibitor.
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7 protocols using cytofix cytoperm plus kit with golgiplug
Multiparametric Flow Cytometry of Tumor-Infiltrating Immune Cells
Multiparametric Analysis of Tumor-Infiltrating Immune Cells
Analysis of immune cell populations from ex vivo tumor cultures was performed using the following antibodies: V500 anti-human CD4, FITC anti-human CD8, PE-Cy7 anti-human CD69, PE-CF594 anti-human CD127, BV421 anti-human Granzyme B, PerCP-Cyanine5.5 anti-human Perforine, APC-Cyanine7 anti-human CD107a antibodies (Supplementary table S2). Intracellular staining was performed using BD Cytofix/Cytoperm Plus Kit (with BD GolgiPlug) (BD Biosciences, New Jersey, USA), according to manufacturer’s instructions. Samples were stained after Fc blocking using Human TruStain FcX Receptor Blocking Solution (Biolegend, California, USA).
Analysis of immune cells from murine PDX-OvCa tumors was performed using the following antibodies: Alexa Fluor 700 anti-human CD3, V500 anti-human CD4, FITC anti-human CD8, PE-Cy7 anti-human CD69 (Supplementary table S2).
All samples were acquired in duplicates using FACS Aria II cell sorter (BD Biosciences, New Jersey, USA) collecting at least 50000 events per sample. Data analysis was performed using Flowjo software v10 (Flowjo LLC, BD Biosciences, New Jersey, USA).
Single-cell immune profiling by flow cytometry
Flow Cytometric Analysis of IL-4+ CD4+ T Cells
Multiparameter Flow Cytometry Analysis
Characterizing Lung Immune Cells
Cytokine Profiling of Monocytes
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