Abts and potassium persulfate

Manufactured by Merck Group

Sourced in Germany, Chile

ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) and potassium persulfate are laboratory reagents commonly used in colorimetric assays. ABTS is a chromogenic substrate that undergoes a color change upon oxidation, which can be used to quantify the presence or activity of oxidizing agents. Potassium persulfate is an oxidizing agent that can be used in conjunction with ABTS to facilitate this color-changing reaction. The specific details of their intended use and applications would require further information.

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Potassium persulfate (671 citations) ABTS TM (2 citations)

2 protocols using abts and potassium persulfate

Standardization of Shuxuening Injection

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Ten reference compounds including clitorin, rutinum, isoquercitin, quercetin-3-O-D-glucosyl]-(1-2)-L-rhamnoside, kaempferol 3-rutinoside, kaempferol-7-O-β-D- glucopyranoside, apigenin-7-O-Glucoside, quercetin-3-O-[2-O-(6-O-p-hydroxyl-E-couMaroyl) -D-glucosyl]-(1-2)-L-rhaMnoside, quercetin, 3-O-{2-O-[6-O-(p-hydroxyl-E-coumaroyl)-glucosyl]-(1-2)rhamnosyl} kaempferol were purchased from Chengdu Must Bio. Sci. and Tec. Co. Ltd. (Chengdu, China). 20 batches of Shuxuening injection were obtained from Shineway Pharmaceutical Co. Ltd. (Shijiazhuang, China). A Milli-Q Academic ultra-pure water system purchased from Milford (Millipore, USA) supplied deionized water which was utilized for the preparation of sample and buffer solution. Acetonitrile (ACN) and methanol (MeOH) were from Merck (Chromatographic, Germany) and ABTS and potassium persulfate were from Sigma (Analytical, USA). The other chemicals were analytical reagents.

Ma H., Li J., An M., Gao X.M, & Chang Y.X. (2018). A powerful on line ABTS+–CE-DAD method to screen and quantify major antioxidants for quality control of Shuxuening Injection. Scientific Reports, 8, 5441.

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UHPLC-MS Analysis of Flavonoids

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UHPLC-MS Solvents, LC-MS formic acid and reagent grade chloroform were from Merck (Santiago, Chile). Ultrapure water was obtained from a Millipore water purification system (Milli-Q Merck Millipore, Chile). HPLC standards, (kaempferol, quercetin, isorhamnetin, eriodictyol, luteolin, apigenin, naringenin, all standards with purity higher than 95 % by HPLC) were purchased either from Sigma Aldrich (Saint Louis, Mo, USA), ChromaDex (Santa Ana, CA, USA), or Extrasynthèse (Genay, France). Folin-Ciocalteu phenol reagent (2N), reagent grade Na₂CO₃, AlCl₃, HCl, FeCl₃, NaNO₂, NaOH, quercetin, trichloroacetic acid, sodium acetate, Gallic acid, Trolox, ABTS and potassium persulfate, xanthine oxidase and DPPH (1,1-diphenyl-2-picrylhydrazyl radical) were purchased from Sigma-Aldrich Chemical Co. (Santiago, Chile).

Echiburu-Chau C., Pastén L., Parra C., Bórquez J., Mocan A, & Simirgiotis M.J. (2017). High resolution UHPLC-MS characterization and isolation of main compounds from the antioxidant medicinal plant Parastrephia lucida (Meyen). Saudi Pharmaceutical Journal : SPJ, 25(7), 1032-1039.

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