Gdna eraser treatment

Manufactured by Takara Bio

Sourced in China

The GDNA eraser treatment is a laboratory equipment product designed to remove genomic DNA (gDNA) from samples. It functions by effectively eliminating gDNA contamination, allowing for accurate downstream analysis and processing.

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Lab products found in correlation

Trizol, by Thermo Fisher Scientific (2 mentions) Lightcycler 480 sybr green 1 master mix, by Roche (1 mentions) Primescript rt reagent kit, by Takara Bio (1 mentions) Rnaprep pure plant kit, by Tiangen Biotech (1 mentions) Nanodrop 2000c spectrophotometer, by Thermo Fisher Scientific (1 mentions) Lightcycler 480 2 system, by Roche (1 mentions) Mx3005p, by Agilent Technologies (1 mentions)

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GDNA Eraser (796 citations)

3 protocols using gdna eraser treatment

RNA Extraction and qRT-PCR Analysis

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Total RNA was isolated using RNAprep Pure Plant kit (TIANGEN, Beijing, China). To avoid degradation all steps were carried out at low temperature. RNA quantity and quality was determined using both Nanodrop2000-C spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA) and gel electrophoresis. Approximately 1 μg of qualified total RNA of each sample was used to synthesize first strand complementary DNA according to the manufacturer’s instructions of the PrimeScript® RT Reagent kit after gDNA Eraser treatment (TaKaRa, Dalian, China).
Gene-specific primers for qRT-PCR were designed using the NCBI Primer-Blast Tool. Detailed information of all the primers used in this study is listed in Additional file 8: Table S4. qRT-PCR analysis was performed on a LightCycler TM 480 II System (Roche Applied Science, Basel, Switzerland) using LightCycler 480 SYBR green 1 Master mix (Roche). The relative amounts of candidate genes were calculated with the 2^-ΔΔCT method. The cotton histone3 (GenBank: AF024716.1/locus_ID of NAU-NBI, v1.1: Gh_D03G0370) gene was used as the reference gene. All qRT-PCRs were performed in triplicate.

Yu D., Qanmber G., Lu L., Wang L., Li J., Yang Z., Liu Z., Li Y., Chen Q., Mendu V., Li F, & Yang Z. (2018). Genome-wide analysis of cotton GH3 subfamily II reveals functional divergence in fiber development, hormone response and plant architecture. BMC Plant Biology, 18, 350.

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Quantification of Neurogenic Factors in Brain Samples

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As previously reported 36, 38 , brain samples were dissected and prepared after selection. Separate tissue samples were stored immediately in TRIzol (Invitrogen), and RNA was extracted according to the manufacturer's directions. Genomic DNA was removed by gDNA eraser treatment (Takara), and 1.0 μg of RNA was used for first-strand complementary DNA (cDNA) synthesis (Takara). Quantitative reverse transcriptase PCR (qRT-PCR) was performed on a Stratagene Mx3000P thermal cycler using Universal qRT-PCR master mix for the indicated genes (Takara). The following primers were designed and synthesized:
NRG1, sense 5′-ACCAGCCATCTCATAAAGTGCG-3′, antisense 5′-TTGACGGGTTTGACAGGTCC-3′; ErbB2, sense 5′-TTGGTGGGCAGGTAGGT-GAGTT-3′, antisense 5′-CCTGCCAGTCCCGAGACCCACCT-3′; ErbB3, sense 5′-GTCTGTGTGACCCACTGCAACT-3′, antisense 5′-GGGTGGCAGGAGAAG-CATT-3′; ErbB4, sense 5′-AGTGGTCTGTCATTGCTTATCCTC-3′, antisense 5′-CTGTTGTCCGTGATGTAGATATTGC-3′; BDNF, sense 5′-AAAACCA TAAG-GACGCGGACTT-3′, antisense 5′-GAGGCTCCAAAGG CACTTGA-3′; GDNF, sense 5′-TGACTCCAATATGCCTGAAG ATTATC-3′, antisense 5′-TCAGTCTTT-TAATGGTGGCTTGAA-3′; IGF-1, sense 5′-CCCGTCCCTATCGACAAACA-3′, antisense 5′-TTCCTGCACTTCCTCTACTTGTGT-3′; VEGF, sense 5′-GCAGGCTGCTACGATGA-3′, antisense 5′-TT GATCCGCATGATCTGCAT-3′; FGF-2, sense 5.

Wu Q.Y., Lin L.H., Lu K., Deng S.F., Li W.M., Xu Y., Zhang B, & Liu J.H. (2024). Astrocytic 5-HT_1A receptor mediates age-dependent hippocampal LTD and fear memory extinction in male mice. Experimental & molecular medicine.

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Quantifying Brain Region-Specific Gene Expression

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As previously reported^{34 (link), 35 (link)}, mouse brain samples were dissected and prepared after selection. To make preparations for the PCR, we dissected the mice brain 24 h after the anxiety test. The cortex (mainly from piriform cortex neighboring the BLA, −1.5 mm posterior to the bregma) and amygdala (mainly from BLA or mainly from CeA) were separated from frozen sections. Different brain tissue was placed immediately in Trizol (Invitrogen), and RNA was extracted according to the manufacturer’s manual. Genomic DNA was removed by gDNA eraser treatment (Takara), and 1 mg RNA was used for first-strand cDNA synthesis (Takara). For real-time RT-qPCR, an SYBR detection system (Takara), specific primers (ET1-F: 5′-TGCTGTTCGTGACTTTCC-3′ and ET1-R: 5′-TGTTGACCCAGATGATGTC-3′; ETAR-F:5′-GCTGGTTCCCTCTTCACTTAAGC-3′; ETAR-R:3′-TCATGGTTGCCAGGTTAATGC-5′; ETBR-F:5′-AAGATTGGTGGCTGTTCAGTTTCT-3′; ETBR-R:3′-GAGCATTTCGCAGGTCATCA-5′), and 2 ml of undiluted cDNA were used in 20 ml PCR reactions. Each reaction was performed in duplicate. All real-time RT-PCR reactions were performed in 40 cycles on the iCycler (Agilent Technologies Stratagene Mx3005 P). The relative gene expression and statistical analysis were determined using the Relative Expression Software Tool.

Chen M., Yan H.H., Shu S., Pei L., Zang L.K., Fu Y., Wang Z.F., Wan Q, & Bi L.L. (2017). Amygdalar Endothelin-1 Regulates Pyramidal Neuron Excitability and Affects Anxiety. Scientific Reports, 7, 2316.

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