Endogro basal media

Manufactured by Merck Group

EndoGRO basal media is a cell culture medium designed for the growth and maintenance of endothelial cells. It provides a basic nutrient formulation to support endothelial cell proliferation and function.

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Lab products found in correlation

Axio imager m2, by Zeiss (1 mentions) In vitro angiogenesis assay kit, by Merck Group (1 mentions) μ slide angiogenesis chamber, by Ibidi (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Picogreen assay, by Thermo Fisher Scientific (1 mentions) Fibronectin from human plasma, by Merck Group (1 mentions) Taqman primer, by Thermo Fisher Scientific (1 mentions) Human fibronectin, by Merck Group (1 mentions) Tak 242, by Merck Group (1 mentions) Rneasy plus mini kit, by Qiagen (1 mentions) Quantiteck reverse transcription kit, by Qiagen (1 mentions)

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EndoGRO (16 citations)

3 protocols using endogro basal media

HUVEC Tube Formation Assay

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The HUVEC tube formation assay was performed in a μ-Slide Angiogenesis chamber (ibidi GmbH) using in vitro angiogenesis assay kit (Millipore) following the manufacturer’s instruction. HUVECs (1 × 10⁴) were seeded into each well and treated with AS (500 nM), ISM (1 μM), FN (10 μg/ml) and the endosome-destabilizing peptide L17E (40 µM) in 50 μl of EndoGRO basal media (Millipore) containing 2% fetal bovine serum (FBS) for 6 h before imaging. To study the role of NHERF1/SNAP25 in the anti-angiogenic function of AS/ISM, HUVECs were transfected with shRNA and 48 h later were treated as indicated. The images were recorded by Axio Imager M2 (Carl Zeiss AG) and the total tubular length was quantified by ImageJ.

Chen M., Qiu T., Wu J., Yang Y., Wright G.D., Wu M, & Ge R. (2018). Extracellular anti-angiogenic proteins augment an endosomal protein trafficking pathway to reach mitochondria and execute apoptosis in HUVECs. Cell Death and Differentiation, 25(11), 1905-1920.

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Peptide Effects on HUVEC Adhesion

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The effect of peptide and PAs on HUVEC adhesion was assessed. Fibronectin from human plasma (Sigma) was dissolved in sterile MilliQ water and was added to tissue culture-treated 96 well plates at 5 ug/cm2, which were then left to dry overnight at room temperature in a sterile laminar flow hood. Coated plates were stored at 4 °C and warmed to 37 °C prior to use. Subconfluent HUVECs were incubated for 2 hr in starvation media (EndoGRO basal media from Millipore supplemented with 0.5% fetal bovine serum and 1× penicillin-streptomycin from Gibco) before detaching with 0.05% trypsin/EDTA. Cells were then spun down to remove trypsin/EDTA and were resuspended in PBS containing peptide or PA molecules. Cells were then incubated for 30 min at 37 °C and then plated at 15,000 cells/well. After 45 mins, wells were washed once with PBS to detach loosely adhered cells. A PicoGreen assay (Invitrogen) was used to quantify number of cells in each well. Cell counts were normalized to that obtained in wells with no treatment. Statistical analysis was performed in GraphPad Prism software using one-way analysis of variance (ANOVA) with a Bonferroni's Multiple Comparisons post-test comparing all pairs of columns. The number of samples for all treatment conditions, n, was equal to 4.

Zha R.H., Sur S., Boekhoven J., Shi H.Y., Zhang M, & Stupp S.I. (2014). Supramolecular Assembly of Multifunctional Maspin-Mimetic Nanostructures as a Potent Peptide-Based Angiogenesis Inhibitor. Acta biomaterialia, 12, 1-10.

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Studying Amyloid-beta Effects on HBMEC

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Human brain microvascular endothelial cells (HBMEC) from Cell System Corporation were used to study the effects of amyloid β fragments. The cells were maintained with EndoGRO‐basal media (Millipore) plus EndoGRO MV‐VEGF supplement kit (Millipore), on human fibronectin (Sigma) coated culture dish. Amyloid β protein fragment 1–40 (Aβ40, Sigma, A1075) was dissolved with 1% acetic acid and then incubated at 37°C for 4 days before use. TAK‐242 (Sigma), a cell‐permeable cyclohexenecarboxylate that disrupts TLR4 interaction with adaptor molecules by selectively binding to TLR4 intracellular residue Cys747, was dissolved with DMSO. For treatment, HBMEC before passage 15 were seeded in 12‐well plates precoated with human fibronectin. Before confluent, HBMEC were exposed to Aβ40 (1 µM) for 3 days, without or with TAK‐242 (2 µM). Total RNAs were prepared from cells after treatment with RNeasy Plus Mini kit (Qiagen, 74134). Corresponding cDNA were synthesized by QuantiTeck reverse transcription kit (Qiagen, 205311). The expressions of related genes were measured with Taqman primers (Applied Biosystems) on QuantStudio 3.0, using 2^−ΔΔCt method.

Deng W., Guo S., van Veluw S.J., Yu Z., Chan S.J., Takase H., Arai K., Ning M., Greenberg S.M., Lo E.H, & Bacskai B.J. (2022). Effects of cerebral amyloid angiopathy on the brain vasculome. Aging Cell, 21(8), e13503.

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