The microglial BV-2 cell line was seeded at 1.2 x 104 density on coverslip properly lodge in 6 multiwells in their complete growth medium. The following day, the medium was replaced with starvation medium supplemented with appropriate stimuli. After each treatment, the medium was discarded and a previously used standard procedure was followed.12 The primary antibody (rabbit anti-cytochrome C, rabbit anti-Iba1, rabbit anti-Nrf2, or mouse anti-CD86; SantaCruz Biotechnology, Santa Cruz, CA, USA), diluted 1:200 in blocking solution were used. The images were acquired by a motorized 654 Leica DM6000 B microscope equipped with a DFC350FX camera (Leica, Mannheim, Germany).
Rabbit anti cytochrome c
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Rabbit anti-cytochrome C is an immunological reagent used for the detection and quantification of cytochrome C, a key protein involved in cellular respiration and apoptosis. It is a polyclonal antibody raised in rabbits against cytochrome C.
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Lab products found in correlation
2 protocols using rabbit anti cytochrome c
1
Microglial BV-2 Cell Immunocytochemistry
2
Apoptosis Pathway Antibodies Analysis
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The following primary antibodies were used in this study: rabbit anti-Rack1 and mouse anti-FEM1b antibodies (Abcam), mouse anti-caspase 9 antibody (Cell Signaling), rabbit anti-active caspase 9 (Abcam), mouse anti-caspase 3 and rabbit anti-active caspase 3 antibodies (Abcam), rabbit anti-cytochrome c (Santa Cruz), mouse anti-β-actin or GAPDH, and rabbit anti-Apaf-1 antibodies (Sigma), and mouse anti-PKCα or PKCβ antibody (Santa Cruz).
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