Ab71286

Manufactured by Abcam

Sourced in China, United Kingdom

Ab71286 is a lab equipment product offered by Abcam. It serves as an analytical tool for research purposes. The core function of this product is to facilitate the analysis and investigation of specific biological or chemical samples.

Automatically generated - may contain errors

Lab products found in correlation

P h3 antibody, by Santa Cruz Biotechnology (1 mentions) Sc 8656 r, by Merck Group (1 mentions) Ab64264, by Abcam (1 mentions) Orb182397, by Biorbyt (1 mentions) Ab6658, by Abcam (1 mentions) Ab10805, by Abcam (1 mentions) Sc 20701, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 488 568 633, by Thermo Fisher Scientific (1 mentions) Ab821, by Evrogen (1 mentions) Sc 101526, by Santa Cruz Biotechnology (1 mentions) Sp8 lightning confocal microscope, by Leica (1 mentions) Ab155421, by Abcam (1 mentions) Tcs sp8, by Leica (1 mentions) Alexa fluor 647 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)

5 protocols using ab71286

Immunohistochemical Analysis of Zebrafish Embryos

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The embryos were fixed in 4% PFA (PBS) for 1 h at room temperature. After washing in PBST (0.1% Tween 20 in PBS), the tails of embryos were clipped for genomic DNA extraction for genotyping, and the rest parts were mounted with 1.5% agarose dissolved in 30% sucrose PBS and then equilibrated overnight at 4°C. The blocks were mounted with OCT compound (Sakura). The sections were cut serially to an 11-μm thickness and collected on poly-L-lysine coated glass slides (CITOGLAS, 188105). Immunofluorescence staining was performed as described (Guan et al., 2016 (link)). Rabbit polyclonal antibody against zebrafish Fabp10a (1:500) and mouse monoclonal antibody against zebrafish Bhmt (1:500) were generated by Hangzhou HuaAn Biotechnology Company. P-H3 antibody was purchased from Santa Cruz (sc-8656-R, 1:600), PCNA antibody from Sigma (P8825, 1:1000), and 2F11 monoclonal antibody from Abcam (ab71286, 1:500). Alexa Fluor 647-labeled secondary antibody was used for visualization.

Gao C., Huang W., Gao Y., Lo L.J., Luo L., Huang H., Chen J, & Peng J. (2018). Zebrafish hhex-null mutant develops an intrahepatic intestinal tube due to de-repression of cdx1b and pdx1. Journal of Molecular Cell Biology, 11(6), 448-462.

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Zebrafish Tissue Preparation and Immunohistochemistry

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Zebrafish embryos were fixed in paraformaldehyde and processed for in situ hybridization using standard protocols (http://zfin.org/ZFIN/Methods/ThisseProtocol.html). Adult zebrafish were fixed in Dietrich’s fixative, paraffin-embedded and serially sectioned at 5–10 μm and stained with hematoxylin and eosin (H&E) using standard techniques. For immunohistochemistry, fixed tissue was embedded in paraffin and serially sectioned for H&E and immunohistochemical analysis. Slides were deparaffinized and rehydrated prior to heat-induced antigen retrieval. Cholangiocarcinoma was visualized using a primary antibody (2F11; Abcam, ab71286) and processed using an HRP/DAB (ABC) detection kit (Abcam, ab64264).

Nissim S., Leshchiner I., Mancias J.D., Greenblatt M.B., Maertens O., Cassa C.A., Rosenfeld J.A., Cox A.G., Hedgepeth J., Wucherpfennig J.I., Kim A.J., Henderson J.E., Gonyo P., Brandt A., Lorimer E., Unger B., Prokop J.W., Heidel J.R., Wang X.X., Ukaegbu C.I., Jennings B.C., Paulo J.A., Gableske S., Fierke C.A., Getz G., Sunyaev S.R., Harper J.W., Cichowski K., Kimmelman A.C., Houvras Y., Syngal S., Williams C, & Goessling W. (2019). Mutations in RABL3 alter KRAS prenylation and are associated with hereditary pancreatic cancer. Nature genetics, 51(9), 1308-1314.

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Whole-Mount Antibody Staining of Zebrafish Larvae

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For whole-mount antibody staining, larvae were removed the skin with the Tweezers, washed several times with PT (PBS + 1% Triton X-100) and incubated with primary antibodies below: Anxa4 (1:1000; ab71286, Abcam, Cambridge, MA), 5-methyl-cytosine (1:100; ab10805, Abcam, Cambridge, MA), Alcam (1:50; zn5, ZIRC, Eugene, OR), Bhmt (1:500, a kind gift from Jinrong Peng, Zhejiang University, China), Dendra2 (1:1000; AB821, Evrogen, Moscow, Russia), pS6 (1:500; 2215, Cell Signaling, MA, USA), p4EBP1 (1:500; 2855, Cell Signaling, MA, USA), Dnmt1 (1:200, sc-20701, Santa Cruz Biotechnology, Santa Cruz, CA, a kind gift from Jingwei Xiong, Peking University, China), GFP (1:1000, ab6658, Abcam, Cambridge, MA), DsRed (1:500, sc-101526, Santa Cruz Biotechnology, Santa Cruz, CA) and Tomato (1:1000; orb182397, Biorbyt, TX, USA), After primary antibody incubation, larvae were washed several times with PT and incubated with secondary antibodies conjugated to Alexa Fluor 488/568/633 (1:1000; Invitrogen, Grand Island, NY). The primary and secondary antibodies were diluted in the blocking solution (PBS + 4% BSA + 1% Triton X-100) and incubated at 4 °C overnight and washed with PT for five times.

He J., Zhou Y., Qian C., Wang D., Yang Z., Huang Z., Sun J., Ni R., Yang Q., Chen J, & Luo L. (2022). DNA methylation maintenance at the p53 locus initiates biliary-mediated liver regeneration. NPJ Regenerative Medicine, 7, 21.

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Immunohistochemistry Imaging of Zebrafish Liver

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At 5–7 dpf, zebrafish were anesthetized in 0.1% tricaine and fixed in 10% formalin overnight at room temperature (RT), and then embedded in paraffin blocks and sectioned at 2–4 nm thickness. Slides were dewaxed through 2 changes of xylene and hydrated through 100%, 90%, and 70% ethanol. Antigen retrieval was performed using a pressure cooker, and then slides were treated with H₂O₂ and methanol for peroxidase inhibition. Blocking was performed using 10% goat serum in PBS plus 0.1% Tween 20 (PBS-T) for 1 hour at RT, and then slides were incubated with a primary antibody for 1 hour, washed for 15 minutes in PBS-T, and incubated 30 minutes in secondary antibody. Slides were mounted using Prolong Diamond and cured at RT for 48 hours before proceeding to imaging on a Leica SP8 Lightning confocal microscope. The following antibodies were used: Mdr1 (Thermo Fisher Scientific, BS-0563R, 1:100); BSEP (MilliporeSigma, HPA019035, 1:100); BSEP (Abcam, ab155421, 1:200); GFP (Abcam, ab252881, 1:100); Rab11 (Genetex, GTX127328, 1:300); and 2F-11 (Abcam, ab71286, 1:100).

Karolczak S., Deshwar A.R., Aristegui E., Kamath B.M., Lawlor M.W., Andreoletti G., Volpatti J., Ellis J.L., Yin C, & Dowling J.J. (2023). Loss of Mtm1 causes cholestatic liver disease in a model of X-linked myotubular myopathy. The Journal of Clinical Investigation, 133(18), e166275.

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Immunofluorescence Analysis of Embryonic Epicardium

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The exposed embryos of each group were collected and fixed in 4% paraformaldehyde (PFA) overnight, and then the pericardial epicardium was peeled off under the microscope with a No. 5 forceps. Anti‐PCNA (1:100, ab71286, Abcam, UK) antibody incubation was performed as described in the previous method.²⁸ Secondary antibodies were incubated using Alexa Fluor 647™ Goat anti‐mouse IgG (1: Invitrogen™). Apoptotic cells were stained using the TUNEL Apoptosis Detection Kit (Alexa Fluor 647) according to the manufacturer's instructions. Nuclei were stained for 30 min using DAPI dye. Finally, the fluorescence was imaged and photographed using a laser scanning confocal microscope (Leica TCS SP8).

Gong F., Shen T., Zhang J., Wang X., Fan G., Che X., Xu Z., Jia K., Huang Y., Li X, & Lu H. (2021). Nitazoxanide induced myocardial injury in zebrafish embryos by activating oxidative stress response. Journal of Cellular and Molecular Medicine, 25(20), 9740-9752.

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