Gelatin sepharose 4b columns

Manufactured by GE Healthcare

Sourced in Sweden

Gelatin sepharose-4B columns are a type of chromatography column used for the separation and purification of biomolecules. The columns are packed with a cross-linked, porous agarose-based matrix that has been further modified with gelatin. This matrix is designed to provide efficient and selective separation of proteins, peptides, and other macromolecules based on their size and charge properties.

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Lab products found in correlation

Fetal calf serum (fcs), by Biowest (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Hek293ft cell line, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Sepharose 4B (96 citations) Gelatin Sepharose 4B (21 citations) Gelatin-Sepharose (9 citations) Sepharose 4B column (3 citations)

2 protocols using gelatin sepharose 4b columns

Generating Fn1 -/- Mouse Kidney Fibroblasts

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Fn1 -/- mouse kidney fibroblasts were generated and cultured as previously described⁽¹¹ (link)
⁾. For experiments, FN was depleted from fetal calf serum using gelatin sepharose-4B columns (GE Healthcare, Uppsala, Sweden), and the culture medium was supplemented with Penicillin-Streptomycin 100 U/ml and, where indicated, TGF- 1 (5 ng/ml). Absence of Mycoplasma sp. contamination was routinely verified by PCR as described elsewhere⁽³¹ (link)
⁾.

Grapa A.I., Efthymiou G., Van Obberghen-Schilling E., Blanc-Féraud L, & Descombes X. (2023). A spatial statistical framework for the parametric study of fiber networks: Application to fibronectin deposition by normal and activated fibroblasts. Biological Imaging, 3, e25.

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Culture and Characterization of HUVECs and Fibroblasts

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Human umbilical vein endothelial cells (HUVECs) were prepared from fresh human umbilical veins and maintained as previously described (Radwanska et al., 2017) . Fn1 fl/fl mouse kidney fibroblasts (MFs) were kindly provided by Dr. Reinhard Fässler [Max Planck Institute, Martinstread, Germany (Sakai et al., 2001) ]. The HEK293FT cell line was from Life Technologies (Saint Aubin, France). All cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) containing L-glutamine (Thermo Fisher Scientific, Waltham, MA, USA) and 10% fetal calf serum (FCS; 5% for signaling experiments) (Biowest, Nuaillé, France) in a humidified incubator at 37 o C with 5% CO2. For experiments, FN was depleted from FCS using gelatin sepharose-4B columns (GE Healthcare, Uppsala, Sweden), and the culture medium was supplemented with Penicillin-Streptomycin 100 U/ml. No coating was performed on culture dishes and coverslips unless otherwise stated. Absence of Mycoplasma sp. contamination was routinely verified by PCR as described elsewhere (Kong et al., 2001) .

Efthymiou G., Radwanska A., Grapa A.I., Beghelli-de la Forest Divonne S., Grall D., Schaub S., Hattab M., Pisano S., Poet M., Pisani D.F., Counillon L., Descombes X., Blanc-Féraud L, & Van Obberghen-Schilling E. (2021). Fibronectin Extra Domains tune cellular responses and confer topographically distinct features to fibril networks. Journal of cell science, 134(4).

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