Male FSL (n = 20) and control-FRL (n = 20) rats were obtained from the colony maintained by AAM at the Karolinska Institutet. Animals were housed individually in Macrolon cages covered with filter tops (Tecniplast, Italy) under a reversed light/dark 12-h cycle (lights out at 9 a.m.). Food and water were available ad libitum, except prior to the novelty suppressed feeding (NSF) tests when food restrictions were applied, as described below. All behavioral tests and drinking measurements were carried out by an experimenter blind to the animal strains in a dark room illuminated by red lights. The Ethical Committee on Animal Research in Stockholm, Sweden approved all experimental procedures (Dnr N163/14). Experiments were carried out in accordance with all relevant guidelines and regulations, and in compliance with the ARRIVE guidelines.
Filter tops
Manufactured by Tecniplast
Sourced in Italy
Filter tops are lab equipment designed to provide a barrier for cell culture vessels, protecting the contents from airborne contaminants while allowing gas exchange. They are made of durable materials and feature a filtered vent to enable airflow.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using filter tops
1
Behavioral Tests on Male Rats
2
Alcohol Consumption in Rodent Models
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Adult post pubertal age‐matched male NMRI mice (8 to 12 weeks old and 25 to 40 g body weight; B&K Universal AB, Sollentuna, Sweden) were used. We have extensive experience with NMRI mice, and they are considered to be a good model for general use and are extensively used in behavioral studies used in psychopharmacology research (Jerlhag et al., 2009 ). All mice were group‐housed and maintained at a 12/12 hour light/dark cycle. Adult post pubertal age‐matched male Rcc Han Wistar rats (Harlan, Horst, Netherlands) were used as they are known to voluntarily consume alcohol using the intermittent access model and thereby reach physiological relevant blood alcohol concentrations (Simms et al., 2008 ). They were housed individually in high Macrolon III cages covered with filter tops (Tecniplast, Varese, Italy) and maintained on a 12‐hour reversed light dark cycle.
All animals were maintained at 20°C with 50% humidity and tap water and food (Normal chow; Harlan Teklad, Norfolk, UK) were supplied ad libitum. These studies were carried out in strict accordance with the recommendations in the Swedish Animal Welfare Act, and all experiments were approved by the Swedish Ethical Committee on Animal Research in Gothenburg.
All animals were maintained at 20°C with 50% humidity and tap water and food (Normal chow; Harlan Teklad, Norfolk, UK) were supplied ad libitum. These studies were carried out in strict accordance with the recommendations in the Swedish Animal Welfare Act, and all experiments were approved by the Swedish Ethical Committee on Animal Research in Gothenburg.
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